四棱豆碱性凝集素的结晶及初步X射线研究

Crystallization and preliminary X-ray studies of the basic lectin from winged bean (Psophocarpus tetragonolobus).

作者信息

Sankaranarayanan R, Puri K D, Ganesh V, Banerjee R, Surolia A, Vijayan M

机构信息

Molecular Biophysics Unit, Indian Institute of Science, Bangalore.

出版信息

J Mol Biol. 1993 Jan 20;229(2):558-60. doi: 10.1006/jmbi.1993.1056.

DOI:10.1006/jmbi.1993.1056

PMID:8429564

Abstract

The basic lectin from winged bean (Psophocarpus tetragonolobus) could be crystallized using polyethyleneglycol (PEG) 4000 (I), PEG 8000 (II) and 2-methylpentane-2,4-diol (MPD) (III) as precipitants. Crystal forms I and II grew in the presence of methyl-alpha-D-galactopyranoside or N-acetylgalactosamine while III grew in the absence of sugar. The three forms have the same space group (P2(1)2(1)2) and similar unit cell dimensions with two dimeric molecules in the asymmetric unit. The unit cell dimensions are a = 156.8 A, b = 89.0 A, c = 73.3 A for I, a = 155.5 A, b = 92.3 A, c = 72.5 A for II and a = 148.3 A, b = 90.7 A, c = 73.8 A for III. The crystals, particularly those grown using PEG 8000, are suitable for high resolution X-ray analysis, which is in progress.

摘要

来自四棱豆（Psophocarpus tetragonolobus）的碱性凝集素可以使用聚乙二醇（PEG）4000（I）、PEG 8000（II）和2-甲基戊烷-2,4-二醇（MPD）（III）作为沉淀剂进行结晶。晶体形式I和II在甲基-α-D-吡喃半乳糖苷或N-乙酰半乳糖胺存在下生长，而III在无糖条件下生长。这三种形式具有相同的空间群（P2(1)2(1)2），并且具有相似的晶胞尺寸，不对称单元中有两个二聚体分子。晶胞尺寸对于I为a = 156.8 Å，b = 89.0 Å，c = 73.3 Å；对于II为a = 155.5 Å，b = 92.3 Å，c = 72.5 Å；对于III为a = 148.3 Å，b = 90.7 Å，c = 73.8 Å。这些晶体，特别是那些使用PEG 8000生长的晶体，适合进行高分辨率X射线分析，目前该分析正在进行中。