Declercq R, Van Aerschot A, Herdewijn P, Van Meervelt L
Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, B-3001 Heverlee, Belgium.
Acta Crystallogr D Biol Crystallogr. 1999 Jan;55(Pt 1):279-80. doi: 10.1107/S0907444998008270. Epub 1999 Jan 1.
Hexitol nucleic acids are oligonucleotides built up from natural nucleobases and a phosphorylated 1,5-anhydrohexitol backbone. The anhydrohexitol oligonucleotide h(GTGTACAC) was synthesized using phosphoramidite chemistry and standard protecting groups. Crystals of h(GTGTACAC) were obtained at either 279 or 289 K by the hanging-drop vapour-diffusion technique using a 24-matrix screen for nucleic acid fragments. The crystals diffract beyond 2.0 A resolution and belong to the hexagonal space group P6222 (or P6422) with unit-cell parameters a = 36.42 and c = 63.33 A.
己糖醇核酸是由天然核碱基和磷酸化的1,5-脱水己糖醇主链构成的寡核苷酸。使用亚磷酰胺化学方法和标准保护基团合成了脱水己糖醇寡核苷酸h(GTGTACAC)。通过悬滴气相扩散技术，使用针对核酸片段的24种基质筛选，在279 K或289 K下获得了h(GTGTACAC)的晶体。这些晶体的衍射分辨率超过2.0 Å，属于六方空间群P6222（或P6422），晶胞参数a = 36.42 Å，c = 63.33 Å。
