体外进化的生物素结合RNA假结的初步晶体学表征。
Preliminary crystallographic characterization of an in vitro evolved biotin-binding RNA pseudoknot.
作者信息
Nix J C, Newhoff A R, Wilson C
机构信息
Department of Biology and Center for the Molecular Biology of RNA, Sinsheimer Laboratories, University of California, Santa Cruz 95064, USA.
出版信息
Acta Crystallogr D Biol Crystallogr. 1999 Jan;55(Pt 1):323-5. doi: 10.1107/S0907444998004934. Epub 1999 Jan 1.
A biotin-binding RNA pseudoknot developed through in vitro selection has been crystallized using the hanging-drop vapor-phase diffusion method. The X-ray diffraction data indicate that the crystals belong to the space group P4222 with unit-cell parameters a = b = 55.2, c = 62.7 A and alpha = beta = gamma = 90 degrees. The crystals are 120 x 80 x 40 microm and VM = 2.17 A3 Da-1. The crystals diffract to 2.8 A.
一种通过体外筛选得到的生物素结合RNA假结，采用悬滴气相扩散法进行了结晶。X射线衍射数据表明，晶体属于空间群P4222，晶胞参数为a = b = 55.2，c = 62.7 Å，α = β = γ = 90°。晶体尺寸为120×80×40微米，VM = 2.17 Å3 Da-1。这些晶体的衍射极限为2.8 Å。
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