Chen B H, Fong J F, Chiang C H
Department of Clinical Laboratory, Kaohsiung Medical College Hospital, Taiwan, Republic of China.
Kaohsiung J Med Sci. 1999 Feb;15(2):87-93.
We collected blood samples from 94 adult non-hematological outpatients and inpatients for complete blood count (CBC) without any flagging at Kaohsiung Medical College Hospital in order to investigate the effect of (1) different anticoagulants with Na2 EDTA vs K3 EDTA (2) the underfilling of blood collection volume (2 ml, 3. 5 ml vs standard 5 ml) (3) the difference in storage stability between 1 hour, 4 hours, 8 hours and 12 hours after venesection at room temperature on some selected hemogram parameters (WBC, RBC, hemoglobin, hematocrit, MCV, MCH, MCHC, platelet, percentage of neutrophil and lymphocyte). The automated hematology analyzer we used was SYSMEX NE-8000, (TOA, Japan). All the EDTA collection vacutainer tubes were supplied by Becton-Dickinson (New Jersey, U. S. A.) with the same lot number. Paired t- test was used for statistics. We found that values of hemoglobin, hematocrit, MCV and lymphocyte percentage collected in Na2 EDTA tubes were significantly higher than those collected in K3 EDTA (P < 0.05 for hemoglobin and lymphocyte percentage, and P all < 0.01 for others), while values of MCHC collected in Na2 EDTA were significantly lower than those collected in K3 EDTA (P < 0.05). For underfilling of blood sample, values of hematocrit and MCV with 2 ml blood volume were significantly lower than those with 5 ml blood volume (both P < 0.01), while values of MCHC with 2 ml blood volume were significantly higher than those with 5 ml blood volume (P < 0.01). When the collection blood volume was increased to 3.5 ml, there were no significant difference between values for 3.5 ml and 5ml blood volume (P all > 0.05). In the storage stability study, there was a significant sequential increase of hematocrit and MCV between 1 hour, 8 hours and 12 hours (P < 0.05 and < 0.01, respectively, for 8 hours, P all < 0.01 for 12 hours). There was also a significant sequential decrease of neutrophil percentage between 1 hour and 4, 8, 12 hours' storage at room temperature (P all < 0.01).
我们在高雄医学院附设医院采集了94名成年非血液科门诊患者和住院患者的血样进行全血细胞计数（CBC），且无任何异常标记，以研究以下因素的影响：（1）使用Na2 EDTA与K3 EDTA这两种不同抗凝剂；（2）采血量不足（2 ml、3.5 ml与标准的5 ml）；（3）静脉穿刺后在室温下1小时、4小时、8小时和12小时储存稳定性的差异，对一些选定的血常规参数（白细胞、红细胞、血红蛋白、血细胞比容、平均红细胞体积、平均红细胞血红蛋白含量、平均红细胞血红蛋白浓度、血小板、中性粒细胞百分比和淋巴细胞百分比）的影响。我们使用的自动血液分析仪是SYSMEX NE - 8000（日本东亚）。所有EDTA采血真空管均由美国新泽西州的贝克顿 - 迪金森公司提供，且批号相同。采用配对t检验进行统计分析。我们发现，Na2 EDTA管中采集的血红蛋白、血细胞比容、平均红细胞体积和淋巴细胞百分比的值显著高于K3 EDTA管中采集的值（血红蛋白和淋巴细胞百分比P < 0.05，其他P均< 0.01），而Na2 EDTA管中采集的平均红细胞血红蛋白浓度的值显著低于K3 EDTA管中采集的值（P < 0.05）。对于采血量不足的情况，2 ml血量的血细胞比容和平均红细胞体积的值显著低于5 ml血量的（均P < 0.01），而2 ml血量的平均红细胞血红蛋白浓度的值显著高于5 ml血量的（P < 0.01）。当采血量增加到3.5 ml时，3.5 ml与5 ml血量的值之间无显著差异（均P > 0.05）。在储存稳定性研究中，血细胞比容和平均红细胞体积在1小时、8小时和12小时之间有显著的顺序性增加（8小时时P < 0.05和< 0.01，12小时时P均< 0.01）。在室温下储存1小时与4小时、8小时、12小时之间，中性粒细胞百分比也有显著的顺序性下降（均P < 0.01）。
