Bourgès-Abella Nathalie H, Geffré Anne, Deshuillers Pierre L, Braun Jean-Pierre D, Trumel Catherine
Department of Functional and Biological Sciences, INP-National Veterinary School of Toulouse, Toulouse, France.
Vet Clin Pathol. 2014 Mar;43(1):24-35. doi: 10.1111/vcp.12119. Epub 2014 Feb 11.
Changes in canine hematology measurements may occur when analyses are delayed due to shipment of specimens to a laboratory.
The purpose of this study was to report changes in hematologic variables in healthy and diseased canine blood measured with a Sysmex XT-2000iV during storage at room temperature for 24 and 48 hours.
EDTA-K3 blood samples from 42 healthy and diseased dogs were measured on a Sysmex XT-2000iV analyzer within one hour of sampling, and after storage for 24 and 48 hours at room temperature in the dark.
Storage caused little or no change in RBC count, HGB concentration and MCH, while there was a moderate increase in HCT, MCV and reticulocytes count, and a moderate decrease in MCHC. Decreased platelet counts by impedance (PLT-I) and optical (PLT-O) measurements were associated with increased mean platelet volume (MPV), platelet-large cell ratio (P-LCR) and platelet distribution width (PDW), including a right shift in the platelet histogram and a dispersion of the platelet dot plot on the scattergram. The total and differential WBC count remained stable except for decreased monocyte counts. In the scatterplots, monocytes shifted into the lymphocyte population after 24 hours, and neutrophil population shifted to the right appearing in the eosinophil gate at 48 hours of storage. The disease status had only a small effect on storage-induced changes, and observed changes had no consequences for clinical decisions.
Blood storage at room temperature was accompanied by moderate variations in some hematologic variables, awareness of which helps in avoiding misinterpretations.
由于将标本运送至实验室而导致分析延迟时，犬类血液学测量值可能会发生变化。
本研究的目的是报告在室温下储存24小时和48小时期间，使用Sysmex XT - 2000iV对健康和患病犬类血液中的血液学变量变化情况。
从42只健康和患病犬采集的乙二胺四乙酸 - K3血液样本，在采样后1小时内，以及在室温黑暗环境中储存24小时和48小时后，使用Sysmex XT - 2000iV分析仪进行测量。
储存对红细胞计数、血红蛋白浓度和平均红细胞血红蛋白含量几乎没有影响或无变化，而血细胞比容、平均红细胞体积和网织红细胞计数有中度增加，平均红细胞血红蛋白浓度有中度降低。通过阻抗法（PLT - I）和光学法（PLT - O）测量的血小板计数降低与平均血小板体积（MPV）、血小板大细胞比率（P - LCR）和血小板分布宽度（PDW）增加相关，包括血小板直方图右移和散点图上血小板点图分散。除单核细胞计数减少外，白细胞总数和分类计数保持稳定。在散点图中，单核细胞在24小时后移入淋巴细胞群体中，中性粒细胞群体在储存48小时时右移并出现在嗜酸性粒细胞区域内。疾病状态对储存诱导的变化影响很小，观察到的变化对临床决策没有影响。
室温下血液储存伴随着一些血液学变量的中度变化，了解这些有助于避免错误解读。
