Lenhoff S, Sallerfors B, Olofsson T
Department of Haematology and Internal Medicine, University Hospital, Lund, Sweden.
Exp Hematol. 1999 Mar;27(3):410-5. doi: 10.1016/s0301-472x(98)00060-5.
In previous studies we characterized the cytokine regulation of granulocyte-macrophage colony-stimulating factor (GM-CSF) and granulocyte colony-stimulating factor (G-CSF) secretion by endothelial cells and monocytes and found differences in secretion pattern within and between these cell systems. In this study, the regulatory effect of T lymphocytes on CSF secretion was examined. T lymphocytes had no effect on CSF secretion by endothelial cells. In contrast, the addition of T lymphocytes significantly and dose dependently downregulated GM-CSF, but not G-CSF, secretion by monocytes. In one of our previous studies it was shown that interleukin-4 (IL-4) and interleukin-10 (IL-10) were the most potent inhibitory cytokines of CSF secretion by monocytes. Both these cytokines are produced by T lymphocytes. However, the downregulating effect on monocyte GM-CSF secretion was not due to increased secretion of T-lymphocyte-derived IL-4 or IL-10. Instead, the presence of T lymphocytes increased the secretion of monocyte-derived IL-10. It was shown earlier than IL-10 regulates CSF secretion by monocytes in an autocrine manner. Our data indicate that T lymphocytes might interfere with this autocrine regulation and thereby influence monocyte function in immune response and cell proliferation.
在以往的研究中，我们对内皮细胞和单核细胞分泌粒细胞-巨噬细胞集落刺激因子（GM-CSF）和粒细胞集落刺激因子（G-CSF）的细胞因子调节进行了表征，并发现这些细胞系统内部和之间的分泌模式存在差异。在本研究中，我们检测了T淋巴细胞对CSF分泌的调节作用。T淋巴细胞对内皮细胞分泌CSF没有影响。相反，添加T淋巴细胞可显著且剂量依赖性地下调单核细胞分泌GM-CSF，但不影响G-CSF的分泌。在我们之前的一项研究中表明，白细胞介素-4（IL-4）和白细胞介素-10（IL-10）是单核细胞分泌CSF的最有效抑制性细胞因子。这两种细胞因子均由T淋巴细胞产生。然而，对单核细胞GM-CSF分泌的下调作用并非由于T淋巴细胞衍生的IL-4或IL-10分泌增加。相反，T淋巴细胞的存在增加了单核细胞衍生的IL-10的分泌。早前有研究表明，IL-10以自分泌方式调节单核细胞分泌CSF。我们的数据表明，T淋巴细胞可能会干扰这种自分泌调节，从而影响单核细胞在免疫反应和细胞增殖中的功能。
