Zhu X Y, Wu C C, Hester P Y
Department of Animal Sciences, Purdue University, West Lafayette, Indiana 47907, USA.
Poult Sci. 1999 Mar;78(3):346-52. doi: 10.1093/ps/78.3.346.
Two experiments were conducted to induce the delayed footpad reaction (DFR) to killed Staphylococcus aureus antigen. In Experiment 1, tracheal, cloacal, and choanal swabs were collected from chickens prior to sensitization with S. aureus to determine the carrier status of S. aureus. The second experiment compared the DFR to the delayed wattle reaction (DWR). Chickens were subjected to single or multiple sensitizations in the neck with S. aureus antigen between 4 and 6 wk of age. One week later, birds were challenged with S. aureus either in the right footpad or wattle. The left footpad or wattle was injected with PBS. The thicknesses of the footpad or the wattle were measured up to 96 h postchallenge. The recoveries of S. aureus from the choanal slit and trachea were significantly higher than that of the cloaca (P < 0.001). Birds of Experiment 1 showed a significant DFR (P < 0.0001) following intradermal challenge with killed S. aureus that was sustained through 48 h postchallenge with no difference in the DFR between carrier and noncarrier birds. In Experiment 2, the thicknesses of the footpad and wattle were significantly increased following challenge with S. aureus (P < 0.0001), with the footpad showing a greater response than the wattle (P < 0.001). Three sensitizing dosages, as compared to two dosages, resulted in a less pronounced DFR and DWR (P < 0.02). These results indicate that the DFR can be used as a delayed reaction model in the study of staphylococcosis in poultry.
进行了两项实验以诱导对灭活金黄色葡萄球菌抗原的足垫迟发反应（DFR）。在实验1中，在用金黄色葡萄球菌致敏之前，从鸡身上采集气管、泄殖腔和后鼻孔拭子，以确定金黄色葡萄球菌的携带状态。第二项实验比较了DFR和肉垂迟发反应（DWR）。在4至6周龄之间，用金黄色葡萄球菌抗原对鸡进行颈部单次或多次致敏。一周后，用金黄色葡萄球菌对鸡的右脚垫或肉垂进行攻毒。在左脚垫或肉垂中注射磷酸盐缓冲盐水（PBS）。在攻毒后长达96小时测量足垫或肉垂的厚度。从后鼻孔裂隙和气管中回收的金黄色葡萄球菌显著高于泄殖腔（P<0.001）。实验1中的鸡在皮内注射灭活金黄色葡萄球菌后表现出显著的DFR（P<0.0001），该反应在攻毒后48小时内持续存在，携带菌和非携带菌的鸡在DFR上没有差异。在实验2中，用金黄色葡萄球菌攻毒后，足垫和肉垂的厚度显著增加（P<0.0001），足垫的反应比肉垂更大（P<0.001）。与两种致敏剂量相比，三种致敏剂量导致的DFR和DWR不太明显（P<0.02）。这些结果表明，DFR可作为家禽葡萄球菌病研究中的迟发反应模型。
