Vaver V A, Todria K G, Prokazova N V, Rozynov B V, Bergelson L D
Biochim Biophys Acta. 1976 Jan 18;486(1):60-9.
A new type of glycolipid has been detected in ripening corn seeds. The presence of ethyleneglycol, galactose, glucose and fatty acids was shown by degradation studies. The products of alkaline deacylation were identified as galactosyl- and glycosylethyleneglycol by thin-layer chromatography and combined gas-liquid chromatography-mass spectrometry. The native ethyleneglycol galactolipid was isolated by distribution of the total lipids between heptane and 95% methanol, following silica gel column chromatography of the methanol soluble fraction. Analysis of the alkaline deacylation products of the isolated ethyleneglycol lipid as well as examination of the mass-spectrum of its tetraacetate showed the new lipid to have the structure of 1-acyl-2-(O-beta-D-galacto-pyranosyl)ethyleneglycol with palmitic, oleic and stearic acids as the main fatty acid components. The fragmentation patterns under electron impact of trimethylsilyl ethers of synthetic ethyleneglycol glycopyranosides and of 1-palmitoyl-2-(O-beta-D-tetraacetylgalactopyranosyl)ethyleneglycol are described.
在成熟的玉米种子中检测到一种新型糖脂。降解研究表明存在乙二醇、半乳糖、葡萄糖和脂肪酸。通过薄层色谱法和气相色谱 - 质谱联用，碱性脱酰产物被鉴定为半乳糖基和糖基乙二醇。在对甲醇可溶部分进行硅胶柱色谱分离后，通过将总脂质在庚烷和95%甲醇之间分配，分离出天然的乙二醇半乳糖脂。对分离出的乙二醇脂质的碱性脱酰产物进行分析以及对其四乙酸酯的质谱进行检测，结果表明这种新脂质具有1 - 酰基 - 2 - (O - β - D - 吡喃半乳糖基)乙二醇的结构，主要脂肪酸成分是棕榈酸、油酸和硬脂酸。描述了合成乙二醇吡喃糖苷的三甲基硅醚和1 - 棕榈酰 - 2 - (O - β - D - 四乙酰半乳糖吡喃糖基)乙二醇在电子轰击下的碎片模式。
