The total lipid was extracted from Staphylococcus lactis I3 with chloroform-methanol mixtures and the glycolipid component was isolated by chromatography on silicic acid. 2. Saponification yielded a non-crystalline glycoside for which the structure O-beta-d-glucopyranosyl-(1-->6)-O-beta-d-glucopyranosyl-(1-->1)-d-glycerol has been established by chemical degradations and by comparison with synthetic material. 3. The role of the glycosyl diglycerides in bacterial membranes is discussed.
摘要
用氯仿 - 甲醇混合物从乳酸链球菌I3中提取总脂质,通过在硅酸上进行色谱分离来分离糖脂成分。2. 皂化反应产生一种非晶态糖苷,通过化学降解并与合成材料比较,确定其结构为O-β - d - 吡喃葡萄糖基 - (1→6) - O-β - d - 吡喃葡萄糖基 - (1→1) - d - 甘油。3. 讨论了糖基二甘油酯在细菌膜中的作用。