Nikushkin E V, Klebanov G I
Biofizika. 1976 Jul-Aug;21(4):619-23.
Temperature-dependent conformational features of human serum (SAH) and egg albumins were studied by fluorescent probes such as 3-metoxybenzantron (MBA), dimethylaminocholkon (DMC) and 1-anilononaphtalene-8-sulfonate (ANS) in temperature range of 4--68 degrees C. It was found that these proteins undergo the conformational transitions accompanied by the changes of the quantrum yield and maximum position of fluorescence of MBA, DMA and ANS at 12--16 degrees C, 16--23 degrees C, 40--62 degrees C for SAH and at 5--25 degrees C, 46--64 degrees C for egg albumin.
利用3-甲氧基苯并蒽酮（MBA）、二甲基氨基胆固醇（DMC）和1-苯胺基萘-8-磺酸盐（ANS）等荧光探针，在4-68摄氏度的温度范围内研究了人血清白蛋白（SAH）和蛋清蛋白的温度依赖性构象特征。结果发现，这些蛋白质会发生构象转变，伴随SAH在12-16摄氏度、16-23摄氏度、40-62摄氏度时以及蛋清蛋白在5-25摄氏度、46-64摄氏度时MBA、DMA和ANS量子产率和荧光最大位置的变化。
