Franco-Zorrilla J M, Fernández-Calvín B, Madueño F, Cruz-Alvarez M, Salinas J, Martínez-Zapater J M
Departamento de Mejora Genética y Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid, Spain.
Plant Mol Biol. 1999 Feb;39(3):427-36. doi: 10.1023/a:1006130629100.
Using the meristems of the cauliflower curd as a source of tissue and a series of subtractive hybridizations and amplification reactions, we have constructed a cDNA library highly enriched in cDNAs expressed in reproductive meristems. The analysis of a sample of 250 clones from this library identified 22 cDNA clones corresponding to genes specifically expressed in these cauliflower meristems. Apart from two clones that corresponded to APETALA1, and two other ones showing similarity to different aminoacyl-tRNA synthetases, the remaining clones showed no similarity to any sequence in the databases and may correspond to novel genes. One of these clones, BoREM1, was further characterized and found to correspond to a gene encoding a protein with features of regulatory proteins that follows a expression pattern very similar to the LEAFY transcripts.
以花椰菜花球的分生组织作为组织来源，通过一系列消减杂交和扩增反应，我们构建了一个在生殖分生组织中表达的cDNA高度富集的cDNA文库。对该文库中250个克隆的样本进行分析，鉴定出22个与这些花椰菜分生组织中特异性表达的基因相对应的cDNA克隆。除了两个与APETALA1相对应的克隆以及另外两个与不同氨酰-tRNA合成酶具有相似性的克隆外，其余克隆与数据库中的任何序列均无相似性，可能对应于新基因。其中一个克隆BoREM1被进一步表征，发现它对应于一个编码具有调节蛋白特征的蛋白质的基因，其表达模式与LEAFY转录本非常相似。
