从花椰菜（甘蓝变种花椰菜）中分离出的flo/lfy同源基因的克隆与序列分析

Cloning and sequence analysis of a flo/lfy homologue isolated from cauliflower (Brassica oleracea L. var. botrytis).

作者信息

Anthony R G, James P E, Jordan B R

机构信息

Department of Molecular Biology, Horticulture Research International, Littlehampton, West Sussex, UK.

出版信息

Plant Mol Biol. 1993 Sep;22(6):1163-6. doi: 10.1007/BF00028986.

DOI:10.1007/BF00028986

PMID:8104529

Abstract

A cDNA library has been constructed from cauliflower curd in which floral development had been initiated. Two cDNAs (pBOFH3 and pBOFH8) have been isolated using the Antirrhinum flo gene as a heterologous probe. The two clones were sequenced and found to contain introns. Comparison of the deduced cDNA sequence of bofh with flo and the Arabidopsis homologue lfy reveals extensive homology. An mRNA transcript of 1.6 kb appears on northern RNA blots. This transcript can be detected, at low levels, before any obvious signs of floral differentiation, reflecting the role bofh plays in determining floral meristem identity.

摘要

已从已启动花发育的菜花球构建了一个cDNA文库。使用金鱼草flo基因作为异源探针分离出两个cDNA（pBOFH3和pBOFH8）。对这两个克隆进行了测序，发现它们含有内含子。将bofh推导的cDNA序列与flo以及拟南芥同源物lfy进行比较，发现有广泛的同源性。在Northern RNA印迹上出现了一条1.6 kb的mRNA转录本。在任何明显的花分化迹象出现之前，就可以检测到这种低水平的转录本，这反映了bofh在决定花分生组织特性中所起的作用。