Van Doorn L J, Figueiredo C, Mégraud F, Pena S, Midolo P, Queiroz D M, Carneiro F, Vanderborght B, Pegado M D, Sanna R, De Boer W, Schneeberger P M, Correa P, Ng E K, Atherton J, Blaser M J, Quint W G
Delft Diagnostic Laboratory, Delft, The Netherlands.
Gastroenterology. 1999 Apr;116(4):823-30. doi: 10.1016/s0016-5085(99)70065-x.
BACKGROUND & AIMS: Distinct allelic types of Helicobacter pylori vacA have been defined. The geographic distribution of vacA alleles and cagA was assessed in this study.
A total of 735 cultures from patients in 24 countries were analyzed by polymerase chain reaction and reverse hybridization on a line probe assay (LiPA).
In 124 (16.9%) of the 735 cultures, multiple vacA genotypes were detected, permitting analysis of 611 strains. In Europe, a distribution gradient of s1 subtypes was observed. In northern and eastern Europe, 89% were subtype s1a. s1a and s1b were equally present in France and Italy, whereas in Spain and Portugal 89% of strains were subtype s1b. s1a and s1b were approximately equally prevalent in North America. In Central and South America, virtually all s1 strains were subtype s1b. Subtype s1c was observed in 77% of the s1 isolates from East Asia. m1 and m2a have equal presence, except on the Iberian peninsula and in Central and South America, where m1 (86.2%) is more prevalent than m2 (13.8%). Subtype m2b was found exclusively among East Asian s1c strains. In all parts of the world, vacA s1/cagA-positive genotypes were associated with peptic ulcer disease (P < 0.001).
These data indicate a geographic distribution of H. pylori genotypes and aid in understanding the relationship of H. pylori with disease.
已明确幽门螺杆菌空泡毒素A（VacA）的不同等位基因类型。本研究评估了VacA等位基因和细胞毒素相关蛋白A（CagA）的地理分布情况。
采用聚合酶链反应和线性探针分析（LiPA）上的反向杂交技术，对来自24个国家患者的735份培养物进行分析。
在735份培养物中的124份（16.9%）中检测到多种VacA基因型，从而能够对611株菌株进行分析。在欧洲，观察到s1亚型的分布梯度。在北欧和东欧，89%为s1a亚型。在法国和意大利，s1a和s1b亚型数量相当，而在西班牙和葡萄牙，89%的菌株为s1b亚型。在北美，s1a和s1b亚型的流行程度大致相同。在中美洲和南美洲，几乎所有s1菌株均为s1b亚型。在东亚分离出的s1菌株中，77%为s1c亚型。m1和m2a亚型数量相当，但在伊比利亚半岛以及中美洲和南美洲除外，在这些地区m1（86.2%）比m2（13.8%）更为流行。m2b亚型仅在东亚s1c菌株中发现。在世界所有地区，VacA s1/CagA阳性基因型均与消化性溃疡疾病相关（P < 0.001）。
这些数据表明幽门螺杆菌基因型存在地理分布差异，有助于理解幽门螺杆菌与疾病的关系。
