Taneja M, Rao K B, Gangawane S, Zawar S G, Totey S M
Embryo Biotechnology Laboratory, National Institute of Immunology, New Delhi, India.
Indian J Exp Biol. 1998 Dec;36(12):1201-8.
Two to four blastomere size biopsies were obtained from each 6-day-old embryo of zebu and crossbred cattle for sex determination. The sex of the embryos was determined with a set of bovine Y-chromosome specific primer pairs by using polymerase chain reaction. Thirty two biopsied embryos after their sex was determined, when transferred fresh to synchronized recipients, resulted in 56.2% pregnancy rate. Sixteen healthy calves were born at full term, while 2 heifers aborted at mid-term from fresh embryo transfer. Simultaneously, 44 biopsied embryos which were kept frozen, were thawed at a later date and transferred to the previously synchronized recipients, thereby leading to 24 pregnancies (54.5%). Twenty-three healthy calves were born at full term, while 1 heifer aborted at mid-term from frozen-thawed embryo transfer. The pregnancy rates from both fresh and frozen-thawed biopsied embryos were comparable with that of controls (P > 0.05). Except for a single misidentification of a male calf as a female by our PCR assay (2.6%), the phenotypic sex of all the live born calves as well as the aborted fetuses was correctly matched with the PCR detection.
从每头6日龄的瘤牛和杂交牛胚胎中获取2至4个卵裂球大小的活检样本用于性别鉴定。通过聚合酶链反应，使用一组牛Y染色体特异性引物对来确定胚胎的性别。32个活检胚胎在确定性别后，直接移植到同期受体中，妊娠率为56.2%。16头健康犊牛足月出生，2头小母牛在新鲜胚胎移植中期流产。同时，44个活检胚胎被冷冻保存，之后解冻并移植到先前同期的受体中，妊娠率为24次（54.5%）。23头健康犊牛足月出生，1头小母牛在冻融胚胎移植中期流产。新鲜和冻融活检胚胎的妊娠率与对照组相当（P>0.05）。除了我们的PCR检测将一头雄性犊牛误鉴定为雌性（2.6%）外，所有出生犊牛以及流产胎儿的表型性别与PCR检测结果均正确匹配。
