Sol M A, Tkaczuk J, Voigt J J, Durand M, Sixou M, Maurette A, Thomsen M
Institut National de la Santé et de la Recherche Médicale, Unit 466, Toulouse, France.
Oral Microbiol Immunol. 1998 Aug;13(4):253-8. doi: 10.1111/j.1399-302x.1998.tb00704.x.
The mechanisms by which the bacterial root-canal infection leads to periapical bone destruction (cysts or granulomas) are not yet well understood. Previous works have shown elements of an active immune response in the lesions. In the present study, flow cytometry was used to improve the characterization of immune cells. Semiquantitative immunohistochemical analysis showed the presence of plasma cells, macrophages and B and T cells. The simultaneous use of several antibodies in flow cytometry allowed a more precise phenotype of the lymphocytes. The cysts displayed an abundance of B lymphocytes at the same time as a relative scarcity of CD8+ cells. CD4+ lymphocytes were the dominant lymphocyte population in most cases. A small number of gamma delta T lymphocytes and natural killer cells was found. These preliminary results show that flow cytometry may be used to characterize immune cells from inflamed tissue and opens the possibility for further functional studies.
细菌根管感染导致根尖周骨破坏（囊肿或肉芽肿）的机制尚未完全明确。以往的研究已表明病变中存在活跃的免疫反应成分。在本研究中，采用流式细胞术来完善免疫细胞的特征描述。半定量免疫组化分析显示存在浆细胞、巨噬细胞以及B细胞和T细胞。流式细胞术中同时使用多种抗体可使淋巴细胞的表型更为精确。囊肿中B淋巴细胞数量丰富，而CD8+细胞相对较少。在大多数情况下，CD4+淋巴细胞是主要的淋巴细胞群体。发现了少量的γδ T淋巴细胞和自然杀伤细胞。这些初步结果表明，流式细胞术可用于表征炎症组织中的免疫细胞，并为进一步的功能研究开辟了可能性。
