Kawashima N, Okiji T, Kosaka T, Suda H
Department of Endodontics, Tokyo Medical and Dental University, Japan.
J Endod. 1996 Jun;22(6):311-6. doi: 10.1016/S0099-2399(96)80266-4.
Quantitative phenotypical analysis for macrophages and lymphoid cells was made on periapical lesions induced by making unsealed pulp exposures in rat molars. Exudative macrophages of diverse morphology were the most dominating immunocompetent cells during all experimental periods. They exhibited almost complete distribution through the periapical lesions by the onset of active lesion expansion, and their kinetics and morphology were considerably similar to Ia+ non-lymphoid cells. Lymphoid cells started to show a notable increase shortly before the onset of active lesion expansion. CD4+ lymphocytes dominated at first, whereas CD8+ lymphocytes and plasma cells were dominant when the lesion size was stabilized. These findings suggest that macrophages of heterogenous populations play essential roles in the lesion pathogenesis by acting, depending on their functional heterogeneity, as effector cells or as antigen-presenting cells that stimulate CD4+ lymphocytes to induce an immune response. Interactions between plasma cells and CD8+ lymphocytes may be related to lesion chronicity.
通过在大鼠磨牙上进行未封闭的牙髓暴露诱导根尖周病变，对巨噬细胞和淋巴细胞进行了定量表型分析。在所有实验阶段，形态各异的渗出性巨噬细胞是最主要的免疫活性细胞。在活跃的病变扩展开始时，它们几乎完全分布于根尖周病变中，其动力学和形态与Ia +非淋巴细胞相当相似。淋巴细胞在活跃的病变扩展开始前不久开始显著增加。起初CD4 +淋巴细胞占主导，而当病变大小稳定时，CD8 +淋巴细胞和浆细胞占主导。这些发现表明，异质性群体的巨噬细胞通过根据其功能异质性作为效应细胞或作为刺激CD4 +淋巴细胞诱导免疫反应的抗原呈递细胞，在病变发病机制中发挥重要作用。浆细胞与CD8 +淋巴细胞之间的相互作用可能与病变的慢性化有关。
