坎帕内利 程序性死亡因子1（PD -1）和程序性死亡因子1配体（PD -L1）在慢性根尖周炎中的表达
CAMPANELLIProgrammed Death 1 (PD-1) and PD-1 Ligand (PD-L1) Expression in Chronic Apical Periodontitis.
作者信息
Delgado Ronan Jacques Rezende, Pinheiro Claudia Ramos, Gasparoto Thaís Helena, Sipert Carla Renata, de Moraes Ivaldo Gomes, Garcia Roberto Brandão, Bramante Clóvis Monteiro, Bernardineli Norberti, Nishiyama Celso Kenji, da Silva João Santana, Torres Sérgio Aparecido, Garlet Gustavo Pompermaier, Campanelli Ana Paula
机构信息
Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, Brazil.
Department of Restorative Dentistry, Discipline of Endodontics, Dental School, University of São Paulo, São Paulo, SP, Brazil.
出版信息
Eur Endod J. 2019 Feb 5;4(1):3-8. doi: 10.14744/eej.2018.46330. eCollection 2019.
OBJECTIVE
This study aimed to examine programmed death protein 1 (PD-1) and programmed death lig-and 1 (PD-L1) expression on leukocytes from chronic apical periodontitis, and to determine the levels of cytokines in the apical periodontitis lesions.
METHODS
Leukocytes from healthy gingival tissue (n=16) and chronic apical periodontitis (n=10) were eval-uated using flow cytometry. The PD-1 and PDL-1 expressions were evaluated using flow cytometry. The cy-tokine levels were evaluated by enzyme-linked immunosorbent assay. Data were analyzed using one-way ANOVA. The statistical significance level was set at P<0.05.
RESULTS
Results showed that the apical periodontitis lesions are more infiltrated by PD-1 and PDL1 lym-phocytes than the control samples. In addition, the PDL-1 expression was detected on macrophages in the apical periodontitis lesions, and was significantly higher compared to leukocytes from healthy gingival tis-sue. The IFN-γ, TGF-β, IL-10, and TNF-α levels were significantly higher in the apical periodontitis lesions com-pared to control samples.
CONCLUSION
The PD-1, PD-L1, and CTLA-4 molecules are evident in apical periodontitis, and can be an impor-tant immune checkpoint in chronic periapical periodontitis.
目的
本研究旨在检测慢性根尖周炎白细胞上程序性死亡蛋白1（PD-1）和程序性死亡配体1（PD-L1）的表达，并测定根尖周炎病变中细胞因子的水平。
方法
采用流式细胞术评估健康牙龈组织（n=16）和慢性根尖周炎（n=10）的白细胞。使用流式细胞术评估PD-1和PDL-1的表达。通过酶联免疫吸附测定法评估细胞因子水平。数据采用单因素方差分析进行分析。统计学显著性水平设定为P<0.05。
结果
结果显示，与对照样本相比，根尖周炎病变中PD-1和PDL1淋巴细胞浸润更多。此外，在根尖周炎病变的巨噬细胞上检测到PDL-1表达，与健康牙龈组织的白细胞相比显著更高。与对照样本相比，根尖周炎病变中IFN-γ、TGF-β、IL-10和TNF-α水平显著更高。
结论
PD-1、PD-L1和CTLA-4分子在根尖周炎中明显，可能是慢性根尖周炎的重要免疫检查点。
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