Babin F, Sarda P, Bouglé D, Billeaud C, Mazette S, Combe N, Nouvelot A, Descomps B, Entressangles B, Crastes de Paulet A, Mendy F
Laboratoire de Biologie et Biochimie des Lipides, Université, Montpellier I, France.
Biol Neonate. 1999 May;75(5):285-93. doi: 10.1159/000014106.
The present multicentric study (three centers) deals with the values of plasma and red blood cell fatty acids obtained in a group of 18 preterm newborns after 2 days (D2), 15 days (D15) and 5 weeks (37th week postconception: 37th wk) of human milk feeding. Analytical methods were randomized between the three centers and quality control was evaluated by repeated analysis of reference samples. 20:4 n-6 varied from 10.71 +/- 1.58% to 9.51 +/- 1.65 and 10.10 +/- 1.42% in plasma phospholipids and from 16.59 +/- 3.30% to 14.68 +/- 3.14 and 18.24 +/- 4.09% in red blood cell phosphatidylethanolamine (RBC-PE) at D2, D15 and 37th wk, respectively, contrasting with the important rise of the precursor (18:2 n-6) in all the fractions studied. In RBC-PE, 22:6 n-3 significantly declined from 3.52 +/- 1. 03% at D2 to 2.56 +/- 0.83% at D15 (p < 0.02) and recovered its initial level at 37th wk (4.08 +/- 1.94%). The recovery of long chain polyunsaturated fatty acid homeostasis at that time was also confirmed by the decline of 16:1 n-7 in cholesterol esters (10.69 +/- 3.92 to 4.32 +/- 2.38%).
本多中心研究（三个中心）涉及一组18名早产新生儿在进行2天（D2）、15天（D15）和5周（孕后第37周：37周）母乳喂养后所获得的血浆和红细胞脂肪酸值。分析方法在三个中心之间随机分配，通过对参考样本的重复分析来评估质量控制。在D2、D15和37周时，血浆磷脂中20:4 n-6分别从10.71±1.58%变化至9.51±1.65%和10.10±1.42%，红细胞磷脂酰乙醇胺（RBC-PE）中则从16.59±3.30%变化至14.68±3.14%和18.24±4.09%，这与所研究的所有组分中前体物质（18:2 n-6）的显著升高形成对比。在RBC-PE中，22:6 n-3从D2时的3.52±1.03%显著下降至D15时的2.56±0.83%（p<0.02），并在37周时恢复到初始水平（4.08±1.94%）。胆固醇酯中16:1 n-7的下降（从10.69±3.92降至4.32±2.38%）也证实了此时长链多不饱和脂肪酸内稳态的恢复。
