硝基还原酶A作为大肠杆菌soxRS调节子的一员受到调控。
Nitroreductase A is regulated as a member of the soxRS regulon of Escherichia coli.
作者信息
Liochev S I, Hausladen A, Fridovich I
机构信息
Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA.
出版信息
Proc Natl Acad Sci U S A. 1999 Mar 30;96(7):3537-9. doi: 10.1073/pnas.96.7.3537.
Abstract
Nitroreductase A catalyzes the divalent reduction of nitro compounds, quinones, and dyes by NADPH. In this paper, nitroreductase A is induced in Escherichia coli by exposure to paraquat in a manner that depends on the expression of soxR. Nitroreductase activity was only slightly induced by paraquat in a strain bearing a mutational defect in the gene encoding nitroreductase A, but it was approximately 3-fold induced in the parental strain. Nitroreductase A thus appears to be a member of the soxRS regulon and probably contributes to the defenses against oxidative stress by minimizing the redox cycling attendant upon the univalent reduction of nitro compounds, quinones, and dyes.
摘要
硝基还原酶A催化NADPH对硝基化合物、醌类和染料的二价还原反应。在本文中，通过百草枯处理，以一种依赖于soxR表达的方式在大肠杆菌中诱导出硝基还原酶A。在编码硝基还原酶A的基因存在突变缺陷的菌株中，百草枯仅轻微诱导硝基还原酶活性，但在亲本菌株中其诱导倍数约为3倍。因此，硝基还原酶A似乎是soxRS调节子的成员之一，并且可能通过最大限度地减少硝基化合物、醌类和染料一价还原伴随的氧化还原循环，从而有助于抵御氧化应激。
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