Marklund L, Nyström P E, Stern S, Andersson-Eklund L, Andersson L
Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Heredity (Edinb). 1999 Feb;82 ( Pt 2):134-41. doi: 10.1038/sj.hdy.6884630.
Quantitative trait loci (QTLs) with large effects on fatness and growth have been identified previously on pig chromosome 4 in an intercross between the European wild pig and Large White domestic pigs. Two F2 sows, heterozygous for the actual chromosome region, were backcrossed to a Large White boar, and two backcross (BC1) boars were in turn backcrossed to Large White/Landrace sows. One of the boars was heterozygous for an intact wild pig-derived QTL region, whereas the other carried a recombinant haplotype. A total of 85 BC2 animals were produced. Phenotypical measurements included daily weight gain, ultrasonic measurements of fat depth at 70 and 90 kg and several carcass traits. QTL segregation was deduced using 15 markers previously assigned to chromosome 4. Highly significant QTL effects were observed on all fatness traits and on the length of the carcass. A small but significant effect on growth was also observed. The results confirm the presence of one or more QTLs on chromosome 4 affecting fatness and growth. There was a good agreement between the estimates of QTL effects in the F2 and BC2 generations. The results from the recombinant sire family allowed us to map the major QTL effect distal to the recombination breakpoint. We propose that this confirmed QTL with a major effect on fatness is designated FAT1.
先前在欧洲野猪和大白家猪的杂交后代中，已在猪的4号染色体上鉴定出对肥胖和生长有重大影响的数量性状基因座（QTL）。将两条在实际染色体区域为杂合子的F2母猪与一头大白公猪回交，然后将两头回交（BC1）公猪再与大白/长白母猪回交。其中一头公猪在完整的野猪来源QTL区域为杂合子，而另一头携带重组单倍型。共产生了85头BC2动物。表型测量包括日增重、70公斤和90公斤时脂肪深度的超声测量以及几个胴体性状。使用先前定位到4号染色体上的15个标记推断QTL分离情况。在所有肥胖性状和胴体长度上均观察到极显著的QTL效应。在生长方面也观察到了较小但显著的效应。结果证实4号染色体上存在一个或多个影响肥胖和生长的QTL。F2和BC2代中QTL效应的估计值之间具有良好的一致性。来自重组父系家族的结果使我们能够将主要QTL效应定位到重组断点的远端。我们建议将这个对肥胖有主要影响的已确认QTL命名为FAT1。
