Oh J D, Kim E S, Lee H K, Song K D
Department of Animal Science, Iowa State University, Ames, IA 50011, USA.
Asian-Australas J Anim Sci. 2015 Nov;28(11):1545-50. doi: 10.5713/ajas.15.0425.
c-MYC (v-myelocytomatosis viral oncogene homologue) is a transcription factor that plays important role in many biological process including cell growth and differentiation, such as myogenesis and adipogenesis. In this study, we aimed to detect MYC gene polymorphisms, their genotype frequencies and to determine associations between these polymorphisms and meat quality traits in Berkshire pigs. We identified a single nucleotide polymorphism (SNP) in intron 2 of MYC gene by Sanger sequencing, i.e., g.3350G>C (rs321898326), that is only found in Berkshire pigs, but not in other breeds including Duroc, Landrace, and Yorkshire pigs that were used in this study. Genotypes of total 378 Berkshire pigs (138 sows and 240 boars) were determined using Hha I restriction enzyme digestion after polymerase chain reaction. Observed allele frequencies of GG, GC, and CC genotypes were 0.399, 0.508, and 0.093 respectively. Statistical analysis indicated that the g.3350G>C polymorphism was significantly associated with pH45min and cooking loss (p<0.05), suggesting that g.3350G>C SNP can be used for pre-selection of pH45min and cooking loss traits in Berkshire pigs.
c-MYC（髓细胞瘤病毒癌基因同源物）是一种转录因子，在包括细胞生长和分化在内的许多生物学过程中发挥重要作用，如肌生成和脂肪生成。在本研究中，我们旨在检测伯克夏猪中MYC基因多态性、它们的基因型频率，并确定这些多态性与肉质性状之间的关联。我们通过桑格测序在MYC基因内含子2中鉴定出一个单核苷酸多态性（SNP），即g.3350G>C（rs321898326），该多态性仅在伯克夏猪中发现，而在本研究中使用的其他品种包括杜洛克猪、长白猪和约克夏猪中未发现。在聚合酶链反应后，使用Hha I限制性内切酶消化确定了总共378头伯克夏猪（138头母猪和240头公猪）的基因型。GG、GC和CC基因型的观察等位基因频率分别为0.399、0.508和0.093。统计分析表明，g.3350G>C多态性与pH45min和蒸煮损失显著相关（p<0.05），这表明g.3350G>C SNP可用于伯克夏猪pH45min和蒸煮损失性状的预选。
