Inoue M, Fujita A, Maeda K
Department of Veterinary Pathology, Faculty of Agriculture, Yamaguchi University, Japan.
Vet Pathol. 1999 Mar;36(2):146-51. doi: 10.1354/vp.36-2-146.
In specific-pathogen-free chickens infected with the highly virulent HPS-2 strain or virulent reference GBF-1 strain of infectious bursal disease virus (IBDV), pathologic changes of the bone marrow were investigated. On histologic examination, bone marrow lesions were prominent in the HPS-2 group but only mild in the GBF-1 group. The bone marrow of the HPS-2 group showed severe lysis and depletion of heterophil myelocytes with pyknotic nuclear alteration 2-3 days after inoculation. On examination with an electron microscope, heterophil myelocytes were characterized by shrinkage of the cytoplasm and peripheral condensation of nuclear chromatin. IBDV particles were not detected in altered myelocytes. A terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end-labeling method demonstrated a positive reaction in only heterophil myelocytes. In contrast, nucleosomal DNA fragmentation in HPS-2-infected bone marrow cells was indiscernible by agarose gel electrophoresis. These findings indicate that lysis of bone marrow cells is selectively induced in heterophil myelocytes at an early stage after IBDV infection and independent of virus replication.
在感染高致病性传染性法氏囊病病毒（IBDV）HPS - 2株或强毒参考GBF - 1株的无特定病原体鸡中，对骨髓的病理变化进行了研究。组织学检查显示，HPS - 2组骨髓病变明显，而GBF - 1组仅为轻度病变。接种后2 - 3天，HPS - 2组骨髓显示严重的嗜异性髓细胞溶解和耗竭，伴有核固缩改变。电子显微镜检查显示，嗜异性髓细胞的特征为细胞质收缩和核染色质周边凝聚。在改变的髓细胞中未检测到IBDV颗粒。末端脱氧核苷酸转移酶介导的脱氧尿苷三磷酸缺口末端标记法仅在嗜异性髓细胞中显示阳性反应。相反，琼脂糖凝胶电泳无法辨别HPS - 2感染的骨髓细胞中的核小体DNA片段化。这些发现表明，IBDV感染后早期，骨髓细胞溶解在嗜异性髓细胞中被选择性诱导，且与病毒复制无关。
