Kimura J, Obata T, Okada H
Endocrinol Jpn. 1976 Oct;23(5):401-6. doi: 10.1507/endocrj1954.23.401.
Resting cells (GO cells) of the uterine surface epithelium in castrated mice began to synthesize DNA with high synchrony from 10 hr after the injection of 50 ng of estradiol-17beta with or without 5.5 mug of clomiphene citrate. Highly synchronized DNA synthesis in GO cells elicited with estradiol was delayed approximately 5 hr when the simultaneous administration of 0.5 mg of progesterone was given. In GO cells of castrated mice which received 5.5 mug of clomiphene or 55 mug of clomiphene plus 50 ng of estradiol, DNA synthesis with partial synchrony began 15 hr after the injection. The effects of estradiol were completely suppressed by the administration of 55 mug of clomiphene. It is suggested that the inhibitory action of clomiphene may be due to the competitive blocking of estrogen binding, while progesterone suppresses the estrogen-induced DNA synthesis of the surface epithelium and transfers them to the GO cell-compartment.
去势小鼠子宫表面上皮的静止细胞（G0细胞）在注射50纳克17β-雌二醇（无论是否添加5.5微克枸橼酸氯米芬）后10小时开始高度同步地合成DNA。当同时给予0.5毫克孕酮时，由雌二醇引发的G0细胞中高度同步的DNA合成延迟约5小时。在接受5.5微克或55微克枸橼酸氯米芬加50纳克雌二醇的去势小鼠的G0细胞中，部分同步的DNA合成在注射后15小时开始。55微克枸橼酸氯米芬的给药完全抑制了雌二醇的作用。提示枸橼酸氯米芬的抑制作用可能是由于竞争性阻断雌激素结合，而孕酮抑制雌激素诱导的表面上皮DNA合成并将它们转移到G0细胞区室。
