Yagita K, Endo T, De Jonckheere J F
Department of Parasitology, National Institute of Infectious Diseases, Tokyo, Japan.
Parasitol Res. 1999 Apr;85(4):284-9. doi: 10.1007/s004360050549.
A total of 90 Acanthamoeba isolates from human eye infections from 15 countries were clustered into distinct genotypes according to their mtDNA restriction fragment patterns. Closely related digestion phenotypes (sequence difference 0.1-1.5%) were integrated into a single genotype, whereas phenotypes with greater than 4.76% difference were considered distinct. Approximately 80% of the human isolates studied fell into 7 of 22 genotypes, indicating that virulence may be associated with specific clusters of cladistic groups of Acanthamoeba. This technique is useful for large-scale surveying of this particular pathogen.
根据线粒体DNA限制性片段模式，对来自15个国家的90株人眼感染棘阿米巴分离株进行聚类分析，确定其不同的基因型。亲缘关系密切的消化表型（序列差异0.1-1.5%）被归为同一基因型，而差异大于4.76%的表型则被视为不同基因型。约80%的被研究人源分离株属于22种基因型中的7种，这表明毒力可能与棘阿米巴进化枝群的特定聚类有关。该技术有助于对这种特殊病原体进行大规模调查。
