Walochnik J, Obwaller A, Aspöck H
Department for Medical Parasitology, Clinical Institute of Hygiene, University of Vienna, 1095 Vienna, Austria.
Appl Environ Microbiol. 2000 Oct;66(10):4408-13. doi: 10.1128/AEM.66.10.4408-4413.2000.
Eleven Acanthamoeba isolates, obtained from Acanthamoeba keratitis patients, from contact lens cases of non-Acanthamoeba keratitis patients, from asymptomatic individuals, from necrotic tissue, and from tap water and two reference strains were investigated by morphological, molecular biological, and physiological means in order to discriminate clinically relevant and nonrelevant isolates. All clinically relevant isolates showed Acanthamoeba sp. group II morphology. 18S ribosomal DNA sequencing revealed sequence type T4 to be the most prevalent group among the isolates and also the group recruiting most of the pathogenic strains. Interestingly, within T4 the strains of no clinical relevance clustered together. Moreover, physiological properties appeared to be highly consistent with initial pathogenicity and with sequence clustering. Altogether, the results of our study indicate a correlation between the phylogenetic relationship and pathogenicity.
从棘阿米巴角膜炎患者、非棘阿米巴角膜炎患者的隐形眼镜病例、无症状个体、坏死组织以及自来水中获取了11株棘阿米巴分离株，并选取了两株参考菌株，通过形态学、分子生物学和生理学方法进行研究，以区分临床相关和不相关的分离株。所有临床相关分离株均呈现棘阿米巴属II组形态。18S核糖体DNA测序显示，序列类型T4是分离株中最普遍的组，也是聚集了大多数致病菌株的组。有趣的是，在T4组内，无临床相关性的菌株聚集在一起。此外，生理特性似乎与初始致病性和序列聚类高度一致。总之，我们的研究结果表明系统发育关系与致病性之间存在关联。
