Martin A, Danforth H D, Jaynes J M, Thornton J E
Embrex Inc., Research Triangle Park, NC 27709, USA.
Parasitol Res. 1999 Apr;85(4):331-6. doi: 10.1007/s004360050557.
This study examined the lytic effect of seven different synthetic peptidyl membrane-interactive molecules (Peptidyl-MIMs) on sporozoites of five different species of Eimeria infecting chickens and merozoites of two different species that infect chickens. All Peptidyl-MIMs (pMIMs) demonstrated antiparasitic effects at concentrations of 1-50 microM during incubation periods varying from 1 to 20 min. In addition, electron microscopy showed that ultrastructural degeneration of the pellicle of sporozoite stages of the parasites occurred within 5-10 min of exposure to 5-microM concentrations of three different pMIMs. Pore-like openings were seen in the pellicle of the sporozoites at the ultrastructural level, which indicated that the pMIMs had the same mechanism of action on the parasites as that reported from studies done on bacteria. A reduction in lesion scores was seen in chickens treated orally with 10-, 50-, or 75-microM concentrations of two different proteolytic stabilized (methylated) pMIMs after challenge with three different species of avian coccidia in battery-cage trials. Collectively these data indicate that pMIMs may be useful in the control of coccidiosis in poultry.
本研究检测了七种不同的合成肽基膜相互作用分子（肽基-MIMs）对感染鸡的五种不同艾美耳球虫的子孢子以及感染鸡的两种不同球虫的裂殖子的裂解作用。在1至20分钟的不同孵育时间内，所有肽基-MIMs（pMIMs）在1至50微摩尔浓度下均表现出抗寄生虫作用。此外，电子显微镜显示，在暴露于5微摩尔浓度的三种不同pMIMs 5至10分钟内，寄生虫子孢子阶段的表膜发生超微结构退化。在超微结构水平上，子孢子的表膜可见类似孔的开口，这表明pMIMs对寄生虫的作用机制与对细菌的研究报道相同。在层笼试验中，用三种不同种类的禽球虫攻击后，口服10、50或75微摩尔浓度的两种不同的经蛋白水解稳定（甲基化）的pMIMs处理的鸡，病变评分降低。总体而言，这些数据表明pMIMs可能有助于控制家禽球虫病。
