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分泌菊粉酶的酿酒酵母菌株产生果糖。

Inulase-secreting strain of Saccharomyces cerevisiae produces fructose.

作者信息

Brevnova E E, Kozlov D G, Efremov B D, Benevolensky S V

机构信息

Institute of Genetics and Selection of Industrial Microorganisms, Moscow 113545, Russia.

出版信息

Biotechnol Bioeng. 1998 Nov 20;60(4):492-7.

PMID:10099455
Abstract

The gene encoding inulase of the yeast Kluyveromyces marxianus (INU1Km) was cloned and expressed in the inulin-negative yeast Saccharomyces cerevisiae. Cells of S. cerevisiae transformed with the INU1Km gene have acquired extracellular inulase activity and were able to grow in the medium with inulin as a sole carbon source. The S. cerevisiae strain was constructed that is capable of heterologous expression of secreted K. marxianus inulase and is defective in fructose uptake due to null-mutations of the hexokinase structural genes HXK1 and HXK2. When grown in inulin-containing media, this strain is capable of accumulating at least 10% glucose-free fructose in the culture liquid.

摘要

对马克斯克鲁维酵母(Kluyveromyces marxianus)的菊粉酶编码基因(INU1Km)进行了克隆,并在不产菊粉酶的酿酒酵母(Saccharomyces cerevisiae)中进行表达。用INU1Km基因转化的酿酒酵母细胞获得了胞外菊粉酶活性,并且能够在以菊粉作为唯一碳源的培养基中生长。构建了酿酒酵母菌株,该菌株能够异源表达分泌型马克斯克鲁维酵母菊粉酶,并且由于己糖激酶结构基因HXK1和HXK2的无效突变而在果糖摄取方面存在缺陷。当在含菊粉的培养基中生长时,该菌株能够在培养液中积累至少10%的无葡萄糖果糖。

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引用本文的文献

1
Identification of hexose kinase genes in Kluyveromyces marxianus and thermo-tolerant one step producing glucose-free fructose strain construction.鉴定马克斯克鲁维酵母中的己糖激酶基因和构建耐热一步法生产无葡萄糖果糖的菌株。
Sci Rep. 2017 Mar 24;7:45104. doi: 10.1038/srep45104.