来自噬菌体λ强启动子的重组基因的表达会触发大肠杆菌中的SOS反应。

The expression of recombinant genes from bacteriophage lambda strong promoters triggers the SOS response in Escherichia coli.

作者信息

Arís A, Corchero J L, Benito A, Carbonell X, Viaplana E, Villaverde A

机构信息

Institut de Biologia Fonamental and Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain.

出版信息

Biotechnol Bioeng. 1998 Dec 5;60(5):551-9. doi: 10.1002/(sici)1097-0290(19981205)60:5<551::aid-bit5>3.0.co;2-m.

DOI:10.1002/(sici)1097-0290(19981205)60:5<551::aid-bit5>3.0.co;2-m

PMID:10099463

Abstract

The production of several non-related heterologous proteins in recombinant Escherichia coli cells promotes a significant transcription of recA and sfiA SOS DNA repair genes. The activation of the SOS system occurs when the expression of plasmid-encoded genes is directed by the strong lambda lytic promoters, but not by IPTG-controlled promoters either at 37 or at 42 degrees C, and it is linked to an extensive degradation of the proteins after their synthesis. The triggering signal for the SOS response could be an important arrest of cell DNA replication observed within the first hour after the induction of recombinant gene expression. The stimulation of this DNA repair system can partially account for the toxicity exhibited by recombinant proteins on actively producing E. coli cells.

摘要

在重组大肠杆菌细胞中生产几种不相关的异源蛋白会促进recA和sfiA SOS DNA修复基因的显著转录。当质粒编码基因的表达由强λ裂解启动子指导时，SOS系统会被激活，但在37℃或42℃时，IPTG控制的启动子不会激活该系统，并且这与蛋白质合成后广泛降解有关。SOS反应的触发信号可能是重组基因表达诱导后第一小时内观察到的细胞DNA复制的重要停滞。这种DNA修复系统的刺激可以部分解释重组蛋白对活跃生产的大肠杆菌细胞所表现出的毒性。