活化的RecA蛋白可能诱导一个基因的表达，该基因不受LexA阻遏物的控制，其功能对于紫外线照射的λ噬菌体的诱变和修复是必需的。
Activated RecA protein may induce expression of a gene that is not controlled by the LexA repressor and whose function is required for mutagenesis and repair of UV-irradiated bacteriophage lambda.
作者信息
Calsou P, Villaverde A, Defais M
机构信息
Laboratoire de Pharmacologie et de Toxicologie Fondamentales, Toulouse, France.
出版信息
J Bacteriol. 1987 Oct;169(10):4816-21. doi: 10.1128/jb.169.10.4816-4821.1987.
Abstract
The activated form of the RecA protein (RecA) is known to be involved in the reactivation and mutagenesis of UV-irradiated bacteriophage lambda and in the expression of the SOS response in Escherichia coli K-12. The expression of the SOS response requires cleavage of the LexA repressor by RecA and the subsequent expression of LexA-controlled genes. The evidence presented here suggests that RecA induces the expression of a gene(s) that is not under LexA control and that is also necessary for maximal repair and mutagenesis of damaged phage. This conclusion is based on the chloramphenicol sensitivity of RecA -dependent repair and mutagenesis of damaged bacteriophage lambda in lexA(Def) hosts.
摘要
已知RecA蛋白的活化形式（RecA）参与紫外线照射的噬菌体λ的再活化和诱变，以及大肠杆菌K-12中SOS应答的表达。SOS应答的表达需要RecA切割LexA阻遏物以及随后LexA控制基因的表达。此处提供的证据表明，RecA诱导一个不受LexA控制的基因的表达，该基因对于受损噬菌体的最大程度修复和诱变也是必需的。这一结论基于lexA（Def）宿主中RecA依赖性修复和受损噬菌体λ诱变对氯霉素的敏感性。
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