Altona C, Van Boom J H, Haasnoot C A
Eur J Biochem. 1976 Dec 11;71(2):557-62. doi: 10.1111/j.1432-1033.1976.tb11145.x.
The proton signals of a 50 mM solution of thymidylyl-(3'-5')-thymidylyl-(3'-5')-2'-deoxyadenosine, d(T-T-A), in 2H2O at 65 degrees C and p2H=7.4, were detected by nuclear magnetic resonance at 360 MHz. Complete assignment of the 21 ribose proton signals was achieved by extensive decoupling experiments and computer simulations. The coupling constant data show that the ribose rings prefer to adopt the S (2'-endo) conformation. The presence of a substantial amount of a right handed helical structure of the d(-T-A) fragment is indicated from chemical shift considerations. The molecule displays a high degree of conformational purity along the sugar-phosphate backbone.
在65℃、p2H = 7.4的重水（2H2O）中，通过360兆赫的核磁共振检测了50毫摩尔/升的胸苷酰基 -（3'-5'）-胸苷酰基 -（3'-5'）-2'-脱氧腺苷，即d（T-T-A）溶液的质子信号。通过广泛的去耦实验和计算机模拟，实现了对21个核糖质子信号的完全归属。耦合常数数据表明，核糖环更倾向于采取S（2'-内型）构象。从化学位移考虑表明，d（-T-A）片段存在大量右手螺旋结构。该分子沿糖 - 磷酸主链表现出高度的构象纯度。
