一种修饰的核糖四核苷三磷酸的构象分析：在500兆赫的核磁共振条件下于水溶液中研究的m6(2)A-U-m6(2)A-U
Conformational analysis of a modified ribotetranucleoside triphosphate: m6(2)A-U-m6(2)A-U studied in aqueous solution by nuclear magnetic resonance at 500 MHz.
作者信息
Hartel A J, Wille-Hazeleger G, van Boom J H, Altona C
出版信息
Nucleic Acids Res. 1981 Mar 25;9(6):1405-23. doi: 10.1093/nar/9.6.1405.
Abstract
The complete and unequivocal assignment of the 24 ribose proton signals of m6(2)A(1)-U(2)-m6(2)(3)-U(4) by means of 500 MHz NMR spectroscopy at 17 degrees C is given. this assignment is based on scrupulous decoupling experiments carries out at various temperatures. Analysis of the observed chemical shifts and coupling constants of the tetramer shows that the two fragments -m6(2)A(3)-U(4) comprising the 3'-end occur mainly in the classical right-handed stack conformation, whereas the 5'-end the -U(2)- residue appears bulged out in favour of a less well-defined stacking interaction between the bases m6(2)A(1)-and -m6(2)A(3)-. Conformational populations about each of the torsional degrees of freedom along the backbone are discussed. A modernized version of pseudorotation analysis is used to delineate the conformational behaviour of the four ribose rings.
摘要
给出了在17℃下通过500MHz核磁共振光谱对m6(2)A(1)-U(2)-m6(2)(3)-U(4)的24个核糖质子信号进行的完整且明确的归属。该归属基于在不同温度下进行的严格去耦实验。对四聚体观察到的化学位移和耦合常数的分析表明，构成3'-末端的两个片段-m6(2)A(3)-U(4)主要以经典的右手堆叠构象存在，而5'-末端的-U(2)-残基则突出，有利于m6(2)A(1)-和-m6(2)A(3)-碱基之间定义不太明确的堆叠相互作用。讨论了沿主链每个扭转自由度的构象群体。使用伪旋转分析的现代化版本来描述四个核糖环的构象行为。
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