插入到泰勒氏小鼠脑脊髓炎病毒中的淋巴毒素基因的表达

Expression of lymphotoxin gene inserted into Theiler's murine encephalomyelitis virus.

作者信息

Obuchi M, Odagiri T, Lizuka H, Ohara Y

机构信息

Department of Microbiology, Kanazawa Medical University, Uchinada, Ishikawa, Japan.

出版信息

Microbiol Immunol. 1999;43(1):83-6. doi: 10.1111/j.1348-0421.1999.tb02377.x.

DOI:10.1111/j.1348-0421.1999.tb02377.x

PMID:10100752

Abstract

Theiler's murine encephalomyelitis virus (TMEV) belongs to the Picornaviridae genus. DA subgroup strains of this virus induce early, non-fatal polioencephalomyelitis followed by demyelination in the spinal cord, with virus persistence. We investigated the use of DA strain as a vector for the introduction of a foreign gene into the central nervous system. Human lymphotoxin (LT) gene was inserted in the L region, the most upstream part of the polyprotein coding region of DA genome. Expression of LT was demonstrated by an immunoblot and an enzyme-linked immunosorbent assay on BHK-21 cells that were infected with the recombinant virus. In addition, the expressed LT showed cytotoxicity against L-929 cells.

摘要

泰勒氏鼠脑脊髓炎病毒（TMEV）属于微小核糖核酸病毒科。该病毒的DA亚组毒株会引发早期、非致命性的脑脊髓灰质炎，随后脊髓出现脱髓鞘现象，并伴有病毒持续存在。我们研究了将DA毒株用作将外源基因导入中枢神经系统的载体。人淋巴毒素（LT）基因被插入到DA基因组多聚蛋白编码区最上游的L区域。通过免疫印迹法以及对感染重组病毒的BHK - 21细胞进行酶联免疫吸附测定，证实了LT的表达。此外，所表达的LT对L - 929细胞显示出细胞毒性。