Al-hazzaa A A, Bowen I D, Birchall M A
School of Pure and Applied Biology, University of Wales Cardiff, Cardiff, Wales, CF1 3TL, U.K.
Cell Biol Int. 1998;22(4):271-6. doi: 10.1006/cbir.1998.0228.
A novel immunocytochemical method is presented for the qualitative detection of DNA fragmentation in apoptosis. Anti-histone antibody is employed to localize exposed nucleosomal histones (H1, H2a, H2b, H3 and H4) rather than tagging the cut ends of fragmenting DNA as in conventional technique. The method was tested on squamous cell carcinoma of the larynx routinely fixed in formaldehyde and embedded in paraffin wax and compared with results obtained employing Apop-Tag kit (Oncor).
本文介绍了一种用于定性检测凋亡中DNA片段化的新型免疫细胞化学方法。该方法采用抗组蛋白抗体来定位暴露的核小体组蛋白（H1、H2a、H2b、H3和H4），而不是像传统技术那样标记断裂DNA的切口末端。该方法在常规用甲醛固定并石蜡包埋的喉鳞状细胞癌上进行了测试，并与使用Apop-Tag试剂盒（Oncor）获得的结果进行了比较。
