Al-Hazzaa A A, Bowen I D
School of Pure and Applied Biology, University of Wales Cardiff, UK.
Histochem J. 1998 Dec;30(12):897-902. doi: 10.1023/a:1003426519047.
A series of techniques based on LR White resin are described, which permit the use of an anti-histone antibody for the in situ localization of DNA fragmentation characteristic of apoptosis at both the light and the electron microscope level. The methods, applied to an untreated squamous carcinoma of the pharynx, allow direct comparison of light microscopic localization of exposed nucleosomal histones using 3,3'-diaminobenzidine (DAB) and silver-enhanced techniques with a colloidal gold-based anti-histone technique at the electron microscope level. Parallel histochemical localization of acid phosphatase activity is also presented.
本文描述了一系列基于LR White树脂的技术，这些技术允许使用抗组蛋白抗体在光学显微镜和电子显微镜水平上原位定位凋亡特有的DNA片段化。将这些方法应用于未经处理的咽鳞状细胞癌，可直接比较使用3,3'-二氨基联苯胺（DAB）的光学显微镜下暴露核小体组蛋白的定位以及电子显微镜水平上基于胶体金的抗组蛋白技术的银增强技术。还展示了酸性磷酸酶活性的平行组织化学定位。
