沙林样和梭曼样有机磷毒剂可激活大鼠脑中的磷脂酶Cγ。

Sarin-like and soman-like organophosphorous agents activate PLCgamma in rat brains.

作者信息

Niijima H, Nagao M, Nakajima M, Takatori T, Matsuda Y, Iwase H, Kobayashi M

机构信息

Graduate School of Medicine, University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo, 113-0033, Japan.

出版信息

Toxicol Appl Pharmacol. 1999 Apr 1;156(1):64-9. doi: 10.1006/taap.1998.8628.

DOI:10.1006/taap.1998.8628

PMID:10101100

Abstract

We report that there is a time-related change in the phospholipase C (PLC) activities of rat brain cytosol and membrane fractions after iv injection of a soman-like or a sarin-like organophosphorous agent (bis(isopropyl methyl)phosphonate [BIMP] and bis(pinacolyl methyl)phosphonate [BPMP]). PLCgamma was activated in the brain cytosol fraction from BPMP-injected rats. The phosphorylating activity of rat brain membrane fractions were enhanced by BPMP treatment. The brain membrane fractions from BPMP-treated rats phosphorylated several proteins, including supposedly PLCgamma in the brain cytosol fraction from control rats in vitro. These results suggest that soman and sarin may stimulate a membrane tyrosine kinase, including growth factor receptors, directly or indirectly.

摘要

我们报告称，静脉注射类梭曼或类沙林有机磷剂（双（异丙基甲基）膦酸酯[BIMP]和双（频哪基甲基）膦酸酯[BPMP]）后，大鼠脑胞质溶胶和膜组分中的磷脂酶C（PLC）活性会发生与时间相关的变化。BPMP注射大鼠的脑胞质溶胶组分中的PLCγ被激活。BPMP处理增强了大鼠脑膜组分的磷酸化活性。BPMP处理大鼠的脑膜组分在体外使几种蛋白质磷酸化，包括对照大鼠脑胞质溶胶组分中推测的PLCγ。这些结果表明，梭曼和沙林可能直接或间接刺激包括生长因子受体在内的膜酪氨酸激酶。