Matkovics B, Sasvári M, Kotormán M, Varga I S, Hai D Q, Varga C
Biological Isotope Laboratory, József Attila University of Szeged, Hungary.
Acta Physiol Hung. 1997;85(3):183-92.
After intravenous administration of alloxan monohydrate (AL) diabetes developed in rats. Forty-eight hours after the injection the animals were sacrificed, their blood was collected in heparin containing tubes and the tissues were dissected and frozen (-70 degrees C) until their homogenization for pro- and antioxidant testing. Our results can be summarised as follows: (i) In the blood hemolysate the lipid peroxidation slightly elevated and the activity of antioxidant enzymes and reduced glutathione decreased. (ii) Similar phenomena could be observed in the different examined organ homogenates. The organs tested for pro- and antioxidant system were as follows: the liver, heart, skeletal muscle, kidney and pancreas. In our present work we attempt to confirm the data in support of the oxidative predominance over antioxidants in oxidative stress of AL diabetic rats.
大鼠静脉注射一水合四氧嘧啶（AL）后患上糖尿病。注射后48小时处死动物，将其血液收集到含肝素的试管中，解剖组织并冷冻（-70摄氏度），直至将其匀浆用于检测促氧化剂和抗氧化剂。我们的结果总结如下：（i）在血液溶血产物中，脂质过氧化略有升高，抗氧化酶活性和还原型谷胱甘肽减少。（ii）在不同检测的器官匀浆中可观察到类似现象。检测促氧化剂和抗氧化系统的器官如下：肝脏、心脏、骨骼肌、肾脏和胰腺。在我们目前的工作中，我们试图证实支持AL糖尿病大鼠氧化应激中氧化作用优于抗氧化作用的数据。
