Gumieniczek Anna, Hopkała Hanna, Wójtowicz Zbigniew, Nikołajuk Justyna
Department of Medicinal Chemistry, Medical University of Lublin, Poland.
Acta Biochim Pol. 2002;49(2):529-35.
The present study was designed to evaluate the oxidative stress-related parameters in alloxan-induced diabetes in rabbits. After 3, 6, 12 and 24 weeks of hyperglycaemia the enzymatic and non-enzymatic factors were measured in heart tissue of diabetic and control groups. Superoxide dismutase and glutathione peroxidase activities and the contents of total sulfhydryl compounds significantly increased at all time intervals. Catalase activity increased initially (after 3 and 6 weeks), decreased after 12 weeks and increased again at the 24th week of the experiment. Glutathione reductase activity increased initially (at 3rd week), decreased below control level after 6 and 12 weeks, then increased again. Ascorbic acid concentration decreased after 3 and 6 weeks, and increased at the 12th and 24th weeks. The level of lipid peroxidation products was reduced after 3, 6 and 12 weeks of the experiment. After 24 weeks it was significantly elevated. These data suggest that hyperglycaemia induces oxidative stress in the heart but the defense mechanisms in the heart tissue are fairly efficacious against oxidative injury.
本研究旨在评估四氧嘧啶诱导的兔糖尿病模型中与氧化应激相关的参数。在高血糖状态持续3、6、12和24周后，对糖尿病组和对照组心脏组织中的酶促和非酶促因素进行了测量。在所有时间间隔内，超氧化物歧化酶和谷胱甘肽过氧化物酶活性以及总巯基化合物含量均显著增加。过氧化氢酶活性在实验开始时（3周和6周后）升高，12周后降低，在第24周时再次升高。谷胱甘肽还原酶活性在实验开始时（第3周）升高，6周和12周后降至对照水平以下，然后再次升高。抗坏血酸浓度在3周和6周后降低，在第12周和第24周时升高。实验3、6和12周后，脂质过氧化产物水平降低。24周后，其水平显著升高。这些数据表明，高血糖会诱导心脏中的氧化应激，但心脏组织中的防御机制对氧化损伤相当有效。
