Pereira-Chioccola V L, Costa F, Ribeirão M, Soares I S, Arena F, Schenkman S, Rodrigues M M
Departmento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo-Escola Paulista de Medicina, Brazil.
Parasite Immunol. 1999 Feb;21(2):103-10. doi: 10.1046/j.1365-3024.1999.00201.x.
Immunization with naked DNA represents an attractive strategy for development of vaccines against a variety of infections including those caused by protozoan parasites. Recently, we have described that immunization with a plasmid containing the trans-sialidase (TS) gene induced protective immunity against Trypanosoma cruzi infection in BALB/c mice. The present study was aimed at examining and comparing the effectiveness of immunization using either plasmid or recombinant delivered antigens in a mouse strain highly susceptible to infection (A/Sn). Two plasmids were generated containing the coding region for the catalytic domain of TS. TS gene was inserted into pcDNA3 vector with or without the coding region for TS signal peptide. These two plasmids were found to be equally immunogenic at inducing antibodies to TS or inhibition of T. cruzi infection. A third plasmid, in which the TS gene was inserted into the vector VR1012, was as immunogenic as the two others. Immunization with a TS recombinant protein in alum generated a significantly higher antibody response as measured by ELISA or inhibition of TS enzymatic activity. Most relevant, this immunization reduced the mortality due to acute infection.
用裸DNA进行免疫接种是开发针对多种感染（包括由原生动物寄生虫引起的感染）疫苗的一种有吸引力的策略。最近，我们描述了用含有转唾液酸酶（TS）基因的质粒进行免疫接种可诱导BALB/c小鼠对克氏锥虫感染产生保护性免疫。本研究旨在检测和比较在高度易感感染的小鼠品系（A/Sn）中使用质粒或重组递送抗原进行免疫接种的效果。构建了两个含有TS催化结构域编码区的质粒。TS基因被插入到带有或不带有TS信号肽编码区的pcDNA3载体中。发现这两个质粒在诱导针对TS的抗体或抑制克氏锥虫感染方面具有同等的免疫原性。第三个质粒，其中TS基因被插入到VR1012载体中，与其他两个质粒具有相同的免疫原性。用明矾中的TS重组蛋白进行免疫接种，通过ELISA或抑制TS酶活性检测，产生了显著更高的抗体反应。最相关的是，这种免疫接种降低了急性感染导致的死亡率。
