Roger L, Maubois J L, Thapon J L, Brule G
Ann Nutr Aliment. 1978;32(2-3):657-69.
Milk and milk by-products with a low lactose content, very interesting from a nutritional and technological point of view, were obtained by the application of the enzymatic membrane reactor technique. A previous separation of the aqueous phase of milk or ultrafiltrate was necessary and realized by ultrafiltration. The enzyme, a commercial beta-galactosidase, was maintained in solution in the retentate part of the membrane reactor. The optimal conditions of the lactose hydrolysis in milk and whey ultrafiltrates were determined. The behaviour of the aqueous phase of milk in membrane reactor, specially of mineral salts, was studied. Three possibilities were proposed to avoid a calcium-phosphate deposit on the surface of (and in) the reactor membranes: a precipitation of calcium salts by heating, a partial demineralization by electrodialysis or ion exchange, a calcium complexation by addition of sodium citrate. A continuous process for the lactose hydrolysis of milk and demineralized whey or milk ultrafiltrate was proposed. The organoleptic quality of low lactose milk, before and after heat treatment, was evaluated by a tasting panel. High sweeting syrup, were obtained by concentration of lactose hydrolyzed and demineralized ultrafiltrates. Nutritional aspects of these products are discussed specially from the toxicological point of view of galactose.
通过应用酶膜反应器技术,获得了乳糖含量低的牛奶及牛奶副产品,从营养和技术角度来看,它们都非常有趣。牛奶或超滤物水相的预先分离是必要的,通过超滤来实现。该酶为市售的β-半乳糖苷酶,保持在膜反应器截留物部分的溶液中。确定了牛奶和乳清超滤物中乳糖水解的最佳条件。研究了牛奶水相在膜反应器中的行为,特别是矿物盐的行为。提出了三种避免磷酸钙在反应器膜表面及内部沉积的可能性:通过加热使钙盐沉淀、通过电渗析或离子交换进行部分脱矿质、通过添加柠檬酸钠使钙络合。提出了一种连续工艺用于牛奶、脱矿质乳清或牛奶超滤物的乳糖水解。由品尝小组评估了热处理前后低乳糖牛奶的感官品质。通过浓缩水解和脱矿质的超滤物获得了高甜度糖浆。特别从半乳糖的毒理学角度讨论了这些产品的营养方面。