Ochiai T, Enoki Y
Jpn J Physiol. 1976;26(4):375-84. doi: 10.2170/jjphysiol.26.375.
In the blood stored in acid-citrate-dextrose solution (ACD blood), the oxygen affinity and red cell 2, 3-diphosphoglycerate (2, 3-DPG) content showed parallel exponential decays with half-lives of 3 to 4 days. In the blood stored in citrate-phosphate-dextrose solution (CPD blood), the two parameters increased during the first 4 days before showing the same decay as that seen in the ACD blood. There was no significant change in the transmembrane pH gradient of the red cells, and thus the intracellular pH at the plasma pH of 7.40 was always in the range of 7.17 +/- 0.02 throughout the period of storage in ACD medium. In both ACD and CPD blood, the Hill exponent n was always normal (approximately 2.8) while the Bohr coefficient (delta log P50/delta pH) rose along with the lapse of time for preservation. The oxygen affinity of the CPD blood was less influenced by the red cell 2, 3-DPG than was that of the ACD blood. This phenomenon was thought to derive from higher concentration of salts within the CPD-stored red cells. The efficiency of blood oxygen transport in ACD and CPD blood was compared.
在储存在酸性枸橼酸盐葡萄糖溶液（ACD血）中的血液中，氧亲和力和红细胞2,3-二磷酸甘油酸（2,3-DPG）含量呈平行指数衰减，半衰期为3至4天。在储存在枸橼酸盐磷酸盐葡萄糖溶液（CPD血）中的血液中，这两个参数在最初4天内升高，然后呈现与ACD血相同的衰减。红细胞的跨膜pH梯度没有显著变化，因此在ACD培养基储存期间，在血浆pH值为7.40时，细胞内pH值始终在7.17±0.02范围内。在ACD血和CPD血中，希尔指数n始终正常（约为2.8），而玻尔系数（δlog P50/δpH）随着保存时间的推移而升高。CPD血的氧亲和力受红细胞2,3-DPG的影响小于ACD血。这种现象被认为源于CPD储存红细胞内较高的盐浓度。比较了ACD血和CPD血中血液氧运输的效率。
