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含有多聚腺苷酸的细胞核14S核糖核蛋白颗粒的蛋白质组成

Protein composition of nuclear 14 S ribonucleoprotein particles containing poly (A).

作者信息

Molnár J, Samarina O P

出版信息

Mol Biol Rep. 1975 Mar;2(1):1-10. doi: 10.1007/BF00357291.

DOI:10.1007/BF00357291
PMID:1128512
Abstract

Nuclear 14 S RNP particles containing poly (A) from Ehrlich ascites carcinoma cells and rat liver were purified by re-sedimentation in sucrose gradients, by Cs2SO4 density gradient centrifugation and by affinity chromatography on a poly (dT)-Sepharose column. Proteins of these RNP particles were electrophoresed in urea and SDS-polyacrylamide gels. RNP particles of ascites carcinoma cells contain two main bands having molecular weights of 51 000 and 69 000 daltons, respectively, and two or three minor components.

摘要

通过在蔗糖梯度中再沉降、硫酸铯密度梯度离心以及在聚（dT）-琼脂糖柱上进行亲和层析，对来自艾氏腹水癌细胞和大鼠肝脏的含有聚（A）的14S核糖核蛋白颗粒进行了纯化。这些核糖核蛋白颗粒的蛋白质在尿素和十二烷基硫酸钠-聚丙烯酰胺凝胶中进行电泳。腹水癌细胞的核糖核蛋白颗粒含有两条主要条带，分子量分别为51000和69000道尔顿，以及两到三个次要成分。

相似文献

1
Protein composition of nuclear 14 S ribonucleoprotein particles containing poly (A).含有多聚腺苷酸的细胞核14S核糖核蛋白颗粒的蛋白质组成
Mol Biol Rep. 1975 Mar;2(1):1-10. doi: 10.1007/BF00357291.
2
Nuclear and polyribosomal ribonucleoprotein particles containing poly (A) in rat liver cells.大鼠肝细胞中含有多聚腺苷酸的核及多核糖体核糖核蛋白颗粒。
Mol Biol Rep. 1976 Nov;3(2):99-104. doi: 10.1007/BF00423222.
3
The poly(A)-containing ribonucleoproteins in the nucleus and the cytoplasm of ehrlich ascites carcinoma cells.艾氏腹水癌细胞细胞核与细胞质中含聚腺苷酸的核糖核蛋白
Acta Biochim Biophys Acad Sci Hung. 1976;11(4):295-303.
4
Purification of nuclear ribonucleoprotein complexes containing poly(adenylic acid).含聚腺苷酸的核糖核蛋白复合物的纯化
Acta Biochim Biophys Acad Sci Hung. 1975;10(4):263-6.
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Nuclear ribonucleoprotein complexes containing U1 and U2 RNA.含有U1和U2 RNA的核糖核蛋白复合体。
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Characterization of proteins associated with nuclear ribonucleoprotein particles by two-dimensional polyacrylamide gel electrophoresis.通过二维聚丙烯酰胺凝胶电泳对与核糖核蛋白颗粒相关的蛋白质进行表征。
Can J Biochem. 1979 Jan;57(1):32-42. doi: 10.1139/o79-004.
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Characterization of polypeptides associated with messenger RNA and its polyadenylate segment in Ehrlich ascites messenger ribonucleoprotein.艾氏腹水信使核糖核蛋白体中与信使核糖核酸及其聚腺苷酸片段相关的多肽的特性分析
J Biol Chem. 1977 May 25;252(10):3525-32.
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Identification of two discrete ribonucleoprotein particles within the monomer population of rat liver nuclear RNPs.在大鼠肝细胞核核糖核蛋白的单体群体中鉴定出两种离散的核糖核蛋白颗粒。
FEBS Lett. 1983 Jan 10;151(1):127-33. doi: 10.1016/0014-5793(83)80357-3.
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A comparative study of the protein components of ribonucleoprotein particles isolated from rat liver and hepatoma nuclei.从大鼠肝脏和肝癌细胞核中分离出的核糖核蛋白颗粒的蛋白质成分的比较研究。
Exp Cell Res. 1973 Jan;76(1):8-14. doi: 10.1016/0014-4827(73)90412-6.
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[Common patterns in the nuclear organization of tumor cells].
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引用本文的文献

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Ultraviolet light-induced crosslinking of HnRNA to informofer proteins in vitro.紫外线诱导的核内不均一RNA（HnRNA）与信息传递蛋白在体外的交联。
Mol Biol Rep. 1982 Mar 31;8(2):111-6. doi: 10.1007/BF00778513.
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HnRNP particles from Drosophila melanogaster cells.来自黑腹果蝇细胞的核不均一核糖核蛋白颗粒
Mol Biol Rep. 1981 Aug 14;7(4):221-5. doi: 10.1007/BF00805756.
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Nuclear and polyribosomal ribonucleoprotein particles containing poly (A) in rat liver cells.大鼠肝细胞中含有多聚腺苷酸的核及多核糖体核糖核蛋白颗粒。

本文引用的文献

1
Poly a blocks in the nuclear ribonucleoprotein complexes, containing pre-mRNA.多聚 A 结合在含有前体 RNA 的核核糖核蛋白复合物中。
Mol Biol Rep. 1973 Dec;1(4):193-9. doi: 10.1007/BF00357641.
2
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
J Biol Chem. 1969 Aug 25;244(16):4406-12.
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Structural organization of nuclear complexes containing DNA-like RNA.含有类DNA RNA的核复合物的结构组织
Mol Biol Rep. 1976 Nov;3(2):99-104. doi: 10.1007/BF00423222.
4
Autodegradation of pre-mRNA containing nuclear ribo-nucleoprotein particles. The effect of autodegradation on the double-stranded RNA sequences and on the protein composition of particles.包含核糖核蛋白颗粒的前体信使核糖核酸的自降解。自降解对双链核糖核酸序列及颗粒蛋白质组成的影响。
Mol Biol Rep. 1976 Nov;3(2):195-202. doi: 10.1007/BF00423235.
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Messenger ribonucleoprotein complexes of cryptobiotic embryos of Artemia salina.卤虫隐生胚胎的信使核糖核蛋白复合体
Nucleic Acids Res. 1977 Mar;4(3):625-39. doi: 10.1093/nar/4.3.625.
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Methylated cap formation by enzymes bound to nuclear informofer particles.
Mol Biol Rep. 1978 Jun 16;4(2):93-6. doi: 10.1007/BF00775967.
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HnRNP particles.异质性核糖核蛋白颗粒
Mol Biol Rep. 1978 Feb 28;4(1):3-8. doi: 10.1007/BF00775172.
J Mol Biol. 1968 Apr 14;33(1):251-63. doi: 10.1016/0022-2836(68)90292-1.
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Addition of polyadenylate sequences to virus-specific RNA during adenovirus replication.在腺病毒复制过程中，向病毒特异性RNA添加聚腺苷酸序列。
Proc Natl Acad Sci U S A. 1971 Nov;68(11):2806-9. doi: 10.1073/pnas.68.11.2806.
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Characterization of the messenger ribonucleoprotein released from reticulocyte polyribosomes by EDTA treatment.通过EDTA处理对从网织红细胞多核糖体释放的信使核糖核蛋白的表征。
Eur J Biochem. 1971 Mar 11;19(2):264-9. doi: 10.1111/j.1432-1033.1971.tb01313.x.
6
The duck-globin messenger-ribonucleoprotein complex. Resistance to high ionic strength, particle gel electrophoresis, composition and visualisation by dark-field electron microscopy.鸭珠蛋白信使核糖核蛋白复合体。对高离子强度的抗性、颗粒凝胶电泳、组成及暗视野电子显微镜观察
Eur J Biochem. 1973 Jul 16;36(2):455-64. doi: 10.1111/j.1432-1033.1973.tb02930.x.
7
Characteristics of the polyadenylic acid segment associated with messenger ribonucleic acid in mouse sarcoma 180 ascites cells.与小鼠肉瘤180腹水细胞中信使核糖核酸相关的聚腺苷酸片段的特征
Biochemistry. 1972 Feb 29;11(5):792-8. doi: 10.1021/bi00755a018.
8
Two proteins are bound to most species of polysomal mRNA.两种蛋白质与大多数多核糖体mRNA物种结合。
Nat New Biol. 1973 Aug 29;244(139):271-4. doi: 10.1038/newbio244271a0.
9
A protein of molecular weight 78,000 bound to the polyadenylate region of eukaryotic messenger RNAs.一种分子量为78,000的蛋白质与真核生物信使核糖核酸的聚腺苷酸区域结合。
Proc Natl Acad Sci U S A. 1973 Mar;70(3):924-8. doi: 10.1073/pnas.70.3.924.
10
Isolation of messenger ribonucleoproteins from mammalian cells.从哺乳动物细胞中分离信使核糖核蛋白。
J Mol Biol. 1974 Jun 25;86(2):451-68. doi: 10.1016/0022-2836(74)90030-8.