Coakley W T, Bater A J, Deeley J O
Biochim Biophys Acta. 1978 Sep 22;512(2):318-30. doi: 10.1016/0005-2736(78)90256-0.
Human erythrocytes develop vesicles by budding when heated to temperatures close to the thermal transition for spectrin. Regularly spaced strings of vesicles also develop if cells heated to 51--54 degrees C are pulled into unstable shapes by flow of liquid between cover slips. These strings of vesicles develop when cells which had attached to the glass are restrained in the flow by a long membrane-bound tether which maintains a connection with the attachment site on the glass. Breakup into regularly spaced vesicles suggests the breakup of a liquid-like cylinder by growth of Rayleigh instabilities. The ratio of length:diameter of the fragments of cylinder on which each disturbance grew ranged from 2.2 to 5.4 to 1 with a peak of 3.2, as measured from scanning electron micrographs. The upper limit of the range is slightly less than the ratio for the disturbance most likely to grow if surface tension and viscosity alone controlled the vesicle formation. Similar vesicle formation when the form-maintaining structures were weakened has been reported in other systems.
当加热到接近血影蛋白热转变的温度时，人类红细胞通过出芽形成囊泡。如果将加热到51 - 54摄氏度的细胞通过盖玻片之间的液体流动拉成不稳定形状，也会形成规则间隔的囊泡串。当附着在玻璃上的细胞被一个长的膜结合系绳限制在流动中时，这些囊泡串就会形成，该系绳与玻璃上的附着位点保持连接。分裂成规则间隔的囊泡表明，类似液体的圆柱体通过瑞利不稳定性的增长而分裂。从扫描电子显微镜照片测量，每个扰动生长的圆柱体片段的长度与直径之比在2.2至5.4比1之间，峰值为3.2。如果仅由表面张力和粘度控制囊泡形成，该范围的上限略小于最可能生长的扰动的比例。在其他系统中也有报道，当维持形状的结构被削弱时会形成类似的囊泡。
