Coakley W T, Nwafor A, Deeley J O
Biochim Biophys Acta. 1983 Jan 19;727(2):303-12. doi: 10.1016/0005-2736(83)90416-9.
It is known that human erythrocytes in saline fragment by development of an unstable surface wave on the cell rim when cells are heated through the denaturation temperature of the structural protein, spectrin. Here the influence of tetracaine on the fragmentation process has been recorded and analysed by video microscopy of cells heated in rectangular glass microcapillaries. The number of waves per cell rim decreases with increasing tetracaine concentration until, at 0.5 mM tetracaine, wave growth on the cell rim is suppressed on most cells and the cells internalize membrane at the cell dimple. The rate constant for the change in the number of waves per cell with increasing tetracaine concentration is 9.6 mM-1 at a heating rate of 0.5 K/s. 50% of heated cells internalize membrane at 0.14 mM tetracaine. When cells are heated rapidly in suspension in test tubes the presence of tetracaine reduces the temperature for 50% haemolysis from 66 degrees C for washed control cells to 60.5 degrees C for cells in 2 mMs tetracaine. Cells heat in microcapillaries in tetracaine concentrations of 3 mM and higher begin to swell before the spectrin denaturation temperature is reached. Cell fusion was observed at and above the spectrin denaturation temperature in cells heated in 3 and 4 mM tetracaine. It was also noted that the morphology of erythrocytes maintained in 3.6 mM tetracaine for times up to 30 min at 37 degrees C or 20 degrees C was strongly dependent on temperature and time.
已知当人红细胞在盐溶液中加热至结构蛋白血影蛋白的变性温度时，细胞边缘会形成不稳定的表面波，从而导致细胞破碎。在此，通过对在矩形玻璃微毛细管中加热的细胞进行视频显微镜观察，记录并分析了丁卡因对破碎过程的影响。随着丁卡因浓度的增加，每个细胞边缘的波数减少，直到在0.5 mM丁卡因时，大多数细胞边缘的波生长受到抑制，细胞在细胞凹陷处内化膜。在加热速率为0.5 K/s时，每个细胞波数随丁卡因浓度增加而变化的速率常数为9.6 mM⁻¹。50%的加热细胞在0.14 mM丁卡因时内化膜。当细胞在试管中悬浮快速加热时，丁卡因的存在将50%溶血的温度从洗涤对照细胞的66℃降至2 mM丁卡因中细胞的60.5℃。在丁卡因浓度为3 mM及更高的微毛细管中加热的细胞，在达到血影蛋白变性温度之前就开始肿胀。在3 mM和4 mM丁卡因中加热的细胞，在血影蛋白变性温度及以上观察到细胞融合。还注意到，在37℃或20℃下，红细胞在3.6 mM丁卡因中保持长达30分钟的形态强烈依赖于温度和时间。
