Deeley J O, Coakley W T
Biochim Biophys Acta. 1983 Jan 19;727(2):293-302. doi: 10.1016/0005-2736(83)90415-7.
The dynamics of morphological change, when human erythrocytes are heated through the spectrin denaturation temperature in the presence of bovine serum albumin, has been studied using differential interference contrast optics and a television video analysis system. Most washed (control) cells developed a wavy disturbance, with an average of 6.6 +/- 0.4 (2 S.E.) waves per cell rim, when heated. The average number of waves per cell rim decreased and the percentage of heated cells showing morphological changes in the dimple region increased with increasing serum albumin concentration, reaching 100% at 1.0 g/l. The change in the dimple region of cells heated in the presence of serum albumin involved the growth of a regular wavy disturbance around the cell dimple rim. The development of the wavy disturbance on the dimple, which resulted in the internalization of membrane, has been examined as an example of an interfacial instability on a biological membrane. Scanning and transmission electron micrographs confirm membrane internalization.
利用微分干涉对比光学和电视视频分析系统，研究了在牛血清白蛋白存在的情况下，将人类红细胞加热至血影蛋白变性温度时的形态变化动力学。大多数洗涤过的（对照）细胞在加热时会出现波浪状扰动，每个细胞边缘平均有6.6±0.4（2个标准误）个波浪。随着血清白蛋白浓度的增加，每个细胞边缘的平均波浪数减少，在酒窝区域出现形态变化的加热细胞百分比增加，在1.0 g/l时达到100%。在血清白蛋白存在下加热的细胞在酒窝区域的变化涉及细胞酒窝边缘周围规则波浪状扰动的生长。酒窝上波浪状扰动的发展导致膜内化，这已作为生物膜界面不稳定性的一个例子进行了研究。扫描和透射电子显微镜照片证实了膜内化。
