Kajita Y, Majima T, Yoshimura M, Hachiya T, Miyazaki T, Ijichi H, Ochi Y
Clin Chim Acta. 1978 Nov 1;89(3):485-92. doi: 10.1016/0009-8981(78)90414-x.
The present paper describes the detection of an autoantibody for glutamic pyruvic transaminase (GPT) in sera of patients with chronic hepatic disorders. In 16 out of 500 patients, the existence of an antibody for pig GPT was demonstrated by the double antibody method, gel filtration and radioimmunoelectrophoresis. The antibody was demonstrated as an immunoglobulin G (IgG) with either polyclonal or monoclonal type (kappa or lambda). The binding portion of IgG with GPT was determined as the fragment Fab, but not Fc of IgG. Because the binding of 125I-pig GPT with the patient's antibody was displaced by human GPT, this antibody may have the characteristic of cross reacting with both pig and human GPT. Although the mechanism of production of the antibody for GPT and the pathological significance of the antibody in chronic hepatic disorders remained obscure, possible inhibition of GPT activity in serum is suggested in the presence of this antibody.
本文描述了慢性肝病患者血清中谷氨酸丙酮酸转氨酶（GPT）自身抗体的检测。在500例患者中的16例中，通过双抗体法、凝胶过滤和放射免疫电泳证实了猪GPT抗体的存在。该抗体被证明是一种免疫球蛋白G（IgG），具有多克隆或单克隆类型（κ或λ）。IgG与GPT的结合部分被确定为IgG的Fab片段，而非Fc片段。由于125I标记的猪GPT与患者抗体的结合可被人GPT取代，这种抗体可能具有与猪和人GPT都发生交叉反应的特性。尽管GPT抗体的产生机制以及该抗体在慢性肝病中的病理意义仍不清楚，但在这种抗体存在的情况下，提示可能会抑制血清中的GPT活性。
