Heilmann E, Bönninghoff E
Schweiz Med Wochenschr. 1976 Dec 11;106(50):1821-3.
Folic acid concentration in serum and erythrocytes was measured by radio assay according to the principle of competitive binding to proteins with 125I as marker. The method yielded a linear regression with readily reproducible results. The mean value in controls was 7.05 -+/ 3.4 ng/ml in serum and 386.8 -+/ 215.1 ng/ml in erythrocytes. In chronic alcoholics the respective values were significantly lower: 4.79 -+/ 2.02 ng/ml for serum and 344.4 -+/ 198.9 ng/ml for erythrocytes. The clinical importance of folic acid estimations arises from the general frequency of folic aicd deficits. Latent folic acid deficiency is associated with lowered folic acid concentrations in serum and normal values in red cells. In manifest folic acid deficiency both concentrations are decreased.
血清和红细胞中的叶酸浓度采用放射分析法测定，依据以125I为标记与蛋白质竞争性结合的原理。该方法得出的线性回归结果易于重现。对照组血清中的平均值为7.05±3.4纳克/毫升，红细胞中的平均值为386.8±215.1纳克/毫升。慢性酗酒者的相应值显著更低：血清为4.79±2.02纳克/毫升，红细胞为344.4±198.9纳克/毫升。叶酸测定的临床重要性源于叶酸缺乏的普遍发生率。潜在的叶酸缺乏与血清中叶酸浓度降低以及红细胞中值正常相关。明显的叶酸缺乏时，两种浓度均降低。
