Varsanovich E A, Usacheva N T, Stan E Ia, Vasil'ev A V, Chernikov M P
Vopr Med Khim. 1976 Sep-Oct;22(5):648-52.
Alteration in pH and addition of urea (final concentration 3 M) did not affect the electrophoretic and chromatographic separation of glycomacropeptide. At the same time, urea (5 M) distinctly increased an amount of beta-component in separation by free electrophoresis. Sedimentation constant SO2OW and molecular weight of glycomacropeptide were equal to 1.35 S and 13000, respectively. Both fractions of glycomacropeptide had similar amino acid composition and contained phosphorus (0.4%). The variations were observed in the carbohydrate components of the molecules. The heavy component contained 5-fold higher concentration of sialic acids (9.7-9.6%), 2-fold higher concentration of galactose (13%) and acetyl galactosamine (5.0-4.0%) as compared with the beta-component (3.1-0.9%, 7.5% and 2.5%, respectively). These alterations in carbohydrate component of glycomacropeptide were suggested to affect the polymeric conformation of the molecule.
pH 的改变以及添加尿素（终浓度 3 M）并不影响糖巨肽的电泳和色谱分离。同时，尿素（5 M）在自由电泳分离中显著增加了β组分的量。糖巨肽的沉降常数 S020W 和分子量分别为 1.35 S 和 13000。糖巨肽的两个组分具有相似的氨基酸组成且都含有磷（0.4%）。在分子的碳水化合物组分中观察到了差异。与β组分（分别为 3.1 - 0.9%、7.5%和 2.5%）相比，重组分中唾液酸的浓度高 5 倍（9.7 - 9.6%），半乳糖（13%）和 N - 乙酰半乳糖胺（5.0 - 4.0%）的浓度高 2 倍。糖巨肽碳水化合物组分的这些改变被认为会影响分子的聚合构象。
