Häcker-Shahin B, Giannitsis D J
Blutspendezentrale des Städtischen Klinikums, Karlsruhe, Germany.
Transfus Sci. 1992 Apr;13(2):261-4. doi: 10.1016/0955-3886(92)90178-J.
In a series of pilot experiments we depleted neocyte-rich red blood cell concentrates of contaminating leucocytes by use of immunomagnetic beads. The beads were coated with monoclonal antibodies directed against the leucocyte common antigen CD 45. The number of cells isolated depended on the dose of beads which also corresponded to the decrease in leucocytes in the neocyte concentrates. The required ratio of beads to target cells was approximately 25:1, the depletion rate of leucocytes was about 96.0-99.8%.
在一系列预实验中，我们使用免疫磁珠去除了富含幼稚红细胞的红细胞浓缩物中的污染白细胞。这些磁珠包被有针对白细胞共同抗原CD 45的单克隆抗体。分离出的细胞数量取决于磁珠的剂量，而磁珠剂量也与幼稚红细胞浓缩物中白细胞数量的减少相对应。磁珠与靶细胞所需的比例约为25:1，白细胞的去除率约为96.0 - 99.8%。
