Häcker-Shahin B, Karl A, Giannitsis D J
Blutspendezentrale, Städtischen Klinikums Karlsruhe.
Beitr Infusionsther. 1991;28:220-2.
In a series of pilot experiments we depleted platelet concentrates from contaminating leukocytes by use of immunmagnetic beads. The beads were coated with monoclonal antibodies directed against the leukocyte/lymphocyte antigens CD 2, CD 19 or CD 45. The amount of isolated cells depended on the dose of the used beads and was analogous to the observed decrease of leukocytes in platelet concentrates. The total amount of necessary beads for a complete leukocyte depletion depends on the grade of leukocyte contamination, which shows a high variation among different platelet preparations.
在一系列预实验中，我们使用免疫磁珠去除了血小板浓缩物中的污染白细胞。这些磁珠包被有针对白细胞/淋巴细胞抗原CD2、CD19或CD45的单克隆抗体。分离出的细胞数量取决于所用磁珠的剂量，并且与血小板浓缩物中观察到的白细胞减少情况相似。完全去除白细胞所需磁珠的总量取决于白细胞污染程度，不同血小板制剂之间的污染程度差异很大。
