Iijima K, Shimoyama N, Shimoyama M, Mizuguchi T
Department of Anesthesiology, Chiba University, School of Medicine, Japan.
J Clin Laser Med Surg. 1991 Oct;9(5):385-9. doi: 10.1089/clm.1991.9.385.
The effect of low-powered He-Ne lasers (red beam, 632.8 nm, 10.2 mW, and green beam, 543.5 nm, 0.44 mW) on hypotonic hemolysis in human red cells was investigated. Red cell solutions (0.9% NaCl, pH 7.00) were centrifuged and the sediments were irradiated with the red or the green beam for 0 (control), 1, 3, 5, 7, 10, 15 and 30 minutes. Each of the 40 irradiated samples were transferred into hypotonic solution (0.405% NaCl, pH 7.00). After leaving the samples at room temperature for 10 minutes, optical density of every suspension was measured spectrophotomechanically at a wavelength of 545 nm to determine hemoglobin content. Hemolysis percentages of the control samples in hypotonic solution were 52.2 +/- 10.7% (mean +/- SD, N = 20) in the red group and 55.0 +/- 12.0% (N = 20) in the green group. Relative values of hemolysis in the hypotonic solution after irradiation with the red or the green beam decreased significantly between the 7- and 30-minute irradiation. These results demonstrate that low-powered He-Ne lasers have a protective effect on red cell membranes from hypotonic hemolysis and stabilize the cell membranes.
研究了低功率氦氖激光（红色光束，632.8纳米，10.2毫瓦，以及绿色光束，543.5纳米，0.44毫瓦）对人红细胞低渗溶血的影响。将红细胞溶液（0.9%氯化钠，pH值7.00）离心，沉淀物用红色或绿色光束照射0（对照）、1、3、5、7、10、15和30分钟。将40个照射后的样品分别转移到低渗溶液（0.405%氯化钠，pH值7.00）中。将样品在室温下放置10分钟后，用分光光度计在545纳米波长处测量每个悬浮液的光密度，以确定血红蛋白含量。低渗溶液中对照组样品的溶血率在红色组为52.2±10.7%（平均值±标准差，N = 20），在绿色组为55.0±12.0%（N = 20）。用红色或绿色光束照射后，低渗溶液中的相对溶血值在照射7至30分钟之间显著降低。这些结果表明，低功率氦氖激光对红细胞膜免受低渗溶血有保护作用，并能稳定细胞膜。
